摘要
目的 观察神经营养因子-3（NT-3）基因修饰嗅鞘细胞-神经干细胞（OECs-NSCs）复合细胞支架自体移植对脑出血大鼠运动功能的影响.方法 建立Wistar大鼠尾状核脑出血模型,完全随机化分为A组：NT-3基因修饰的OECs-NSCs-复合细胞支架移植组;B组：NT3-OECs-NSCs-复合细胞移植组;C组：支架移植组;D组：空白对照组.各组移植后第1、3、7、14和30天进行运动神经功能评分,并经免疫组织化学染色及荧光染色鉴定移植细胞在大鼠脑内的生存、分布及分化情况.结果 A、B组在移植后第7、14、30天神经功能评分分别为（2.12 ±0.12、1.50±0.11、0.52±0.08）、（2.10±0.16、1.79±0.09、0.91±0.10）明显低于C、D组;C组与D组间差异无统计学意义（P＞0.05）.在第14天和第30天,A组评分明显低于B组（P ＜0.05,P＜0.01）.免疫组织化学方法证实A组移植后存活的NSCs数量较多、向损伤区迁徙的细胞较多.结论 NT-3基因修饰OECs-NSCS-复合细胞支架移植治疗能显著改善脑出血大鼠的运动功能,且移植的NSCs具有较强的存活、迁移及分化能力.
Objective To explore the effects of autotransplantation of NT-3 gene modified olfactory ensheathing cell （OEC） and neural stem cell （NSC） complex adhering to collagen protein-heparin sulfate scaffold on motor function of rats with cerebral hemorrhage.Methods The cerebral hemorrhage model was established with caudate nucleus bleeding in Wistar rats.The animals were randomly divided into 4 groups：A （transplantation of NT-3 modified OEC-NSC complex adhering to scaffold）,B （transplantation of NT-3 modified OEC-NSC complex）,C （transplantation of scaffold） and D （blank control group）.The motor function of hind limbs was assessed at Day 1,3,7,14 and 30 post-transplantation respectively.The survival,distribution and differentiation of transplanted cells were tested by immunohistochemistry and fluorescent staining.Results The neurological functional score of group A （2.12 ± 0.12,1.50 ± 0.11,0.52 ±0.08）or B（2.10 ±0.16,1.79 ±0.09,0.91 ±0.10）was obviously inferior to group C/D at Days 7,14 and 30.No significant difference existed between groups C and D （P 〉 0.05）.The scores of A were markedly lower than those of B at Days 14 and 30 （P 〈 0.05,P 〈 0.01）.The numbers of surviving NSCs and cells migrating to focal area after transplantation was much more in group A than those in other groups.Conclusion Autotransplantation of NT-3 gene modified OEC-NSC complex adhering to collagen proteinheparin sulfate scaffold may markedly ameliorate the motor function of cerebral hemorrhagic in rats.And the transplanted NSCs have better capacities of survival,migration and differentiation.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2014年第19期1483-1487,共5页
National Medical Journal of China
基金
国家自然科学基金（81171171,30970991）
山东省自然科学基金（ZR2009CQ028,Y2007C043）
