摘要
为探讨新型支架材料 Ni- Ti、317L合金在食管局部的致纤维化作用 ,按美国 ASTM标准制备 Ni Ti、317L合金的金属浸提液 ;“组织块培养法”原代培养食管壁疤痕的成纤维细胞 (Fb) ,传代后以金属浸提液进行培养 ,分组后分别培养 4、2 4、48、72 h,MTT法检测不同培养时间后 Fb增殖功能的变化 ;按美国 ASTM标准进行 Ni-Ti、317L合金试件的食管壁内包埋实验 ,即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间 ,术后 2、12周取出包埋组织 ,分析试件周围组织的病理变化 ,并进行胶原纤维染色 ,观察纤维形成状况。结果显示 ,Ni Ti、317L金属浸提液可轻度抑制 Fb的增殖功能 ,随培养时间延长抑制作用呈增强趋势 ,Ni Ti的作用略强于 317L ;包埋实验结果也完全符合美国 ASTM标准的要求 ,随包埋时间延长试件表面的纤维膜逐渐变薄 ,纤维积聚呈减少趋势。这表明 Ni Ti、317L合金在体液中的弥散成分不会激活 Fb的增殖与分泌功能 。
This study was conducted to examine the fibrotic effect of Ni-Ti and 317L alloys in esophagus. The extract fluid from Ni-Ti, 317L alloys was made according to the ASTM standards of U. S. A. The Fb of esophageal scar was cultured primarily, then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagus embedding test of Ni-Ti, 317L alloys was made according to ASTM standards of U. S. A. The tissue around the alloys was taken at weeks 2 and 12, and the pathologic changes were analysed. The results showed that Ni-Ti, 317L extract could depress the proliferating function of Fb gently, and the depressing action increased gradually with the culturing time. The result of embedding test was in accord with the ASTM standards of U. S. A. completely; the fibrotic membrane around the NiTi, 317L alloys became thinner with embedding time. These findings suggested that the scattering composition of Ni-Ti, 317L in body fluid might not activate the proliferating and secreting function of Fb, and the two alloys could not lead to fibrosis of esophagus around them.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2001年第1期12-15,共4页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目!(396 70 341)
