摘要
目的:探讨远志对锰中毒小鼠海马学习记忆的影响以及和p-CREB表达的关系。方法:将60只健康雌性成年昆明小鼠随机分为5组,分别为对照组(CG),锰中毒组(MG),锰中毒加远志高剂量组(远志高剂量组,MHG)、锰中毒加远志中剂量组(远志中剂量组,MMG),锰中毒加远志低剂量组(远志低剂量组,MLG)。采用腹腔注射氯化锰的方式制作小鼠锰中毒模型,采用灌胃的方式进行远志给药。用Morris水迷宫实验测试其空间学习和记忆能力,用免疫组织化学的方法检测海马内CA1,CA3区p-CREB的表达。结果:在定位航行实验中,远志高、中、低剂量组与锰中毒组相比,其平均逃避潜伏期下降,差异有统计学意义(P<0.01),而与对照组相比,平均逃避潜伏期变化不大,差异无统计学意义(P>0.05),锰中毒组与对照组比较,逃避潜伏期明显延长,差异有统计学意义(P<0.01)。在空间探索实验中,远志高、中、低剂量组与锰中毒组相比,其穿越平台次数明显增多,差异有统计学意义(P<0.01),而与对照组相比,穿越平台次数变化不大,差异无统计学意义(P>0.05),锰中毒组与对照组相比,穿越平台次数减少,差异有统计学意义(P<0.01)。与锰中毒组相比,远志高、中剂量组海马内pCREB蛋白的表达显著增多,差异有统计学意义(P<0.01),而与对照组相比,差异无统计学意义(P>0.05),远志低剂量组与对照组相比,其蛋白的阳性表达减少,差异有统计学意义(P<0.01)。结论:药物远志能够改善锰中毒小鼠学习记忆能力的机制可能与p-CREB的表达增加有关。
Objective： To study the effect of polygala tenuifolia willd on learning ＆ memory in the hippocampus and relationship of p-CREB expression in manganese poisoning mice. Methods： Healthy adult 60 female Kunming mice were divided into 5 groups, the control group （CG）, the manganese poisoning group （MG）, the manganese poisoning and treated with gastric perfusion of high, medium, low dosage polygala tenuifolia willd groups （ MHG, MMG and MLG）. The model of manganese exposed mice was manufactured by way of intraperitoneal injection of manganese chloride. The ability of learning and memory was tested by Morris water maze. The expression of p-CREB in CA1, CA3 of hippocampus in mice was detected by immunohistoehemistry. Results：In place navigation test, the MHG, MMG, MLG groups compared with MG, the average escape latency decreased, the difference was statistically significant （P 〈 0.01 ）, and compared with CG, the average escape latency change was no statistically significant difference （ P 〉 0.05 ）, compared with CG, escape the incubation period in MG was significantly prolonged, the difference was statistically significant （P 〈 0.01 ）. In spatial probe test, the MHG, MMG, MLG compared with MG, the crossing platform significantly increased, the difference was statistically significant （P 〈 0.01 ）, while compared with CG, the times of crossing platform was no statistically significant difference （P 〉 0.05）. MG compared with CG group, the times of crossing platform was decrease, the difference was statistically significant （P 〈0.01）. Compared with the MG, p-CREB protein expression in hippocampus in MHG, MMG was significantly increased, the difference was statistically significant （P 〈 0.01 ）, while compared with CG, there was no statistically significant difference （ P 〉 0.05 ）, the MLG compared with CG, the protein positive expression was to reduce, the difference was statistically significant （P 〈 0.01 ）. Conclusion：That mechanism of the polygala tenuifolia willd can improve learning ＆ memory ability in the manganese exposed mice might be involved in the increase of expression level of p-CREB.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第3期325-329,共5页
Chinese Journal of Neuroanatomy
基金
广西自然科学基金(2010GXNSFA013157)
关键词
远志
锰中毒
学习记忆
P-CREB
小鼠
polygala tenuifolia willd
manganese poisoning
learning＆ memory
neurogenesis
p-CREB
mouse
