摘要
本研究通过细胞学、形态学以及流式细胞仪分析等方法对粳稻F1代花培植株进行倍性鉴定，结果表明：苗期利用印记液撕取法观察花培植株的气孔形态、大小以及保卫细胞的叶绿体数并辅以形态学鉴定，是粳稻花培植株群体早期倍性快速判别的简单有效的办法。经流式细胞仪测定和染色体计数验证，结果准确可靠。判别标准如下：单倍体粳稻叶片的表皮气孔保卫细胞叶绿体数≤8，长短轴长平均值分别为11．3μm和6．7μm；而叶绿体数6-16，长短轴长平均值为19．8μm和11．2μm的可视为二倍体；气孔叶绿体数主要分布在10～22之间，长短轴长平均值为30．2μm和14．4μm的可视为四倍体。
The ploidy level of the japonica rice regenerated plants drived from anther culture had been determined by methods of cytological examination, morphology observation and DNA flow cytometry. The results are as follows, combined with table tennis blot analysis and morphology observation the chromosome ploidy of japonica rice regenerated plants derived from anther culture can be identified by simply comparing the stomatal size and counting the chloroplast number in stomatal guard cells at seeding stage. The accuracy of this method was reliable and varified with the DNA flow cytometry detecting and chromosome counting. The criterion is： the average length and width of stomatal for a haploid should be 11.3 μm and 6.7 μm, that for diploids should be 19.8 μm and 11.2 μm, tetraploids should be 30.2 μm and 14.4 μm; The chloroplast number in stomatal guard cells for a haploid should not be more than 8, that for diploids should be 6 to 16, and tetraploids should be 10 to 22.
出处
《分子植物育种》
CAS
CSCD
北大核心
2014年第3期537-542,共6页
Molecular Plant Breeding
基金
江苏省农业科技自主创新资金水稻产业技术体系项目(CX(12)1003-4)
江苏省科技支撑计划项目(BE2012-307
BE2012303)
徐州市科技攻关项目(XF12C016)共同资助
关键词
粳稻
花培植株
倍性鉴定
Japonica rice, Regenerated plants derived from anther culture, Ploidy identification
