摘要
建立了一种用于测定蕨类植物中（C1～C10）脂肪酸成分的毛细管电泳检测方法。用含有口-CD的硼砂缓冲溶液为背景电解质，10种脂肪酸在17min内可实现快速、高效的基线分离。各脂肪酸成分在0．07～5．0μmol／L浓度范围内呈良好的线性关系，相关系数产为0．9983～0．9998，保留时间和峰面积的相对标准偏差分别为0．34％～0．52％和1．36％～1．94％，检测限（3倍信噪比）为0．027～0．042μmol／L。该方法准确可靠，在最优化条件下对卷柏实际样品进行的定性定量检测实验，结果较好。
A simple method for the determination of fatty acids（C1 -C10） based on capillary electrophoresis has been developed. A 58. 5 cm × 50 μm i. d. （50 cm effective length） untreated fused-silica capillary was used. Under the established conditions, 10 fatty acid derivatives were well separatedwithin 17 min. The linearity was in the range of 0.07-5.0 μmol/L,and the detection limits（at a signal-to-noise ratio of 3） were in the range of 0. 027-0. 042 μmol/L. The fatty acids from funaria hedw and selaginella samples were determined under the optimum conditions with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
北大核心
2014年第3期441-444,共4页
Journal of Analytical Science
关键词
脂肪酸
柱前衍生
卷柏
Fatty acids Pre-columnderivation Selaginella
