摘要
为研制一种用于检测牛奶中呕吐毒素(deoxynivalenol,DON)残留的快速检测试纸条,试验以人工合成抗原DONOVA和羊抗鼠二抗为原料,包被硝酸纤维素膜;以抗DON单克隆抗体为原料,制备金标抗体,并冻干保存于反应板微孔中,最终制备出可用于检测牛奶中DON残留的胶体金快速检测试纸条。结果显示,该检测试纸条检测限为100μg/L,重复性、特异性均较好,可用于检测牛奶中DON残留。
For development of a rapid test strip for detecting vomitoxin （DON） residue in milk. We used DON-OVA and secondary antibody goat anti-mouse to coat nitrocellulose membrane,and used anti-DON monoclonal antibody to prepare colloidal gold labeled antibody,then lyophilized in micro holes of reaction plate. At last we prepared a rapid test strip that could be used for detecting DON residue in milk. The results showed that the limit of detection of the test strip was 100 μg/L,and the repeatability and the specificity were preferably,so the rapid strip could be used for detecting DON residue in milk.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第6期73-76,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家十二五科技支撑项目(2012BAK17B00)
北京市科技计划专项-科技型中小企业促进(Z13010101440)
