摘要
目的 在氟研究科研课题中应用 GBW氟标准物质验证血清氟测定标准方法 ,评价其实用价值。方法 用 GBW标准物质对 ISE-标准曲线 ,标准添加法 ,线性范围 ,线性关系 ,灵敏度 ,精密度进行校验 ,并对 40 0余份大鼠血清进行测定。结果 该方法的线性范围 :氟离子浓度为 0 .0 2～ 5 .0 m g/ L、 r=0 .9999、b=5 7.8,检测下限为 0 .0 13m g/ L,标准物质的测定值与标准值相符。结论 该测定方法准确、灵敏 ,样品用量小 (仅 0 .4ml) ,操作简便 ,易于推广 ,具有实际应用价值。
Objective Standard method of testing serum fluoride was tested and verified by certified reference material CRM of free fluoride component in cattle serum (GBW 09143~09144).The method was suited for fluoride research to assess its practical value.Methods ISE standard curve,linear relationship,linear range,sensitive and precision were disired result by CRM.400 serum of fluoride in rats were measured.Results The linear ranges of the method were 0.02~5.0 mg/L,r=0.9999,b=57.8,and test lower limit was 0.013 mg/L.The test value was equal to standard value.Conclusions The standard method is accurate,sensitive,convenient and small sample is used;and it is easy to spread.Thus,it has been of practical value.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2001年第2期140-142,共3页
Chinese Jouranl of Endemiology
