摘要
目的探讨支原体、衣原体和抗精子抗体的检测对男性不育症的应用价值。方法采用培养法、免疫层析法、ELISA法,对102例不育男性和42例已育男性进行支原体、衣原体和抗精子抗体的检测及精液常规的分析。根据结果分为单纯支原体或(和)衣原体阳性组71例,单纯抗精子抗体阳性组21例,支原体或(和)衣原体和抗精子抗体阳性组8例,支原体、衣原体和抗精子抗体阴性组44例,对4组的精液常规主要指标进行比较。结果不育男性支原体、衣原体、抗精子抗体阳性率明显高于已育男性(P<0.05);单纯支原体或(和)衣原体阳性组,单纯抗精子抗体阳性组及支原体或(和)衣原体和抗精子抗体阳性组的精子密度、活动率、活力明显低于阴性组,精子的畸形率、液化时间、白细胞数明显高于阴性组,差异有统计学意义(P<0.05)。结论支原体、衣原体的感染及抗精子抗体的产生对精液多项指标有明显影响,精液质量下降,从而引起男性不育症的发生。
Objective To explore the application value of detection of Mycoplasma,Chlamydia and anti-sperm antibodies for male infertility.Methods Culture method,immune chromatography,and enzyme linked immunosorbent assay were adopted for the detection of Mycoplasma,Chlamydia,and anti-sperm antibodies respectively in 102 cases of infertile males and 42 cases fertile males.And the routine semen analysis was proceed as well.All the subjects were divided into 4 groups according to the detection results：simple Mycoplasma or/and Chlamydia positive group（71 cases）,simple anti-sperm antibodies positive group（21 cases）,My-coplasma and Chlamydia or/and anti-sperm antibodies positive group（8 cases）,Mycoplasma,Chlamydia and anti-sperm antibodies negative group（44 cases）.The main indexes of semen routine were compared among 4 groups.Results The positive rates of Myco-plasma,Chlamydia and anti-sperm antibodies in infertile males were significantly higher than those of fertile males （P 〈0.05）.The sperm densities,activity rates,activity of simple Mycoplasma or/and Chlamydia positive group,simple anti-sperm antibodies positive group,and Mycoplasma and Chlamydia or /and anti-sperm antibodies positive group were significantly lower than the negative group,while the sperm malformation rates,liquefaction times,white blood cell counts of the three groups were significantly higher than the negative group（P 〈0.05 ）.Conclusion Mycoplasma,Chlamydia infection and anti-sperm antibodies production have significant effect on the indexes of semen,which cause decline in semen quality and the occurrence of male infertility.
出处
《国际检验医学杂志》
CAS
2014年第11期1412-1414,共3页
International Journal of Laboratory Medicine
关键词
解脲支原体
人型支原体
沙眼衣原体
抗精子抗体
男性不育症
Ureaplasma ureal yticum
Mycoplasrna hominis
Chlamydia trachomatis
anti-sperm antibody
male infertility
