摘要
应用微量热法结合常规分析方法,研究了桂林会仙湿地沼泽底泥和水稻田微生物在800~4 000μg·g-1Cu2+胁迫下的热代谢活性及Cu2+的固定/转化率。结果表明,在w(Cu2+)为800μg·g-1条件下,水稻田和沼泽底泥土壤微生物对Cu2+的固定/转化率分别为44.93%和34.59%,但在w(Cu2+)为4 000μg·g-1时则分别是93.16%和85.13%;Cu2+对水稻田和沼泽底泥土壤微生物代谢活性的半抑制浓度分别为2 043和2 325μg·g-1;水稻田土壤微生物代谢活性低于沼泽底泥,在土壤及其微生物共同作用下Cu2+的固定/转化率随Cu2+浓度递增而升高;在相同条件下,水稻田和沼泽底泥土壤微生物的代谢速率在α=0.05或α=0.01水平上显著相关。湿地土壤用途改变后,土壤微生物在固定/转化Cu2+的作用上发生了明显变化。
Thermal metabolic activity and Cu2＋ fixation/transformation ratio （Z） of microbes in paddy soil and marsh sediment from the Huixian Wetland, Central Guilin, under Cu2＋ （800-4000 μg·g-1 ） stress were investigated with the microcalorimetric method coupled with conventional analytic methods. Results show that under the stress of 800 μg·g-1 Cu2＋, Z of the microbes in the paddy soil and the sediment was 44.93% and 34.59%, respectively, however under the stress of 4000 μg·g-1 Cu2＋, it rose to 93.16% and 85.13%, respectively; the 50% inhibiting concentration of Cu2＋ against the microbes thermal metabolism was 2043 μg·g-1 in the paddy soil and 2325 μg·g-1 in the marsh sediment; metabolic activity of the microbes was obviously lower in the paddy soil than in the the wetland sediment. Z of Cu2＋ increased with Cu2＋ concentration under the joint effect of the soil and its microbes. Under the same conditions, metabolic rates of the microbes in the paddy soil and in the marsh sediment were significantly correlated （P〈0.05，P〈0.01）. All the above findings show that the role of soil microbes in Cu2＋ fixed/transformed has obviously changed as wetlands are reclaimed into paddy soil, and can be used as scientific basis for management of the wetland resources in Guilin.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2014年第3期358-363,共6页
Journal of Ecology and Rural Environment
基金
国家自然科学基金(31060120)
广西科技开发项目(11107021-5-1)
广西教育厅科学技术研究项目(2009-25)
关键词
桂林
会仙湿地
水稻田
沼泽底泥
微生物热代谢活性
Cu2+固定
转化率
Guilin
Huixian Wetland
paddy land
marsh sediment
thermal metabolic activity of microbe
fixation/transformation of Cu2＋
