摘要
目的:观察乳络通胶囊(乳络通)对实验性大鼠乳腺增生病的影响,探讨其作用机制。方法:Wistar大鼠随机分为6组:正常组、模型组、乳康片(0.53g/kg)和乳络通高、中、低剂量组(0.60g/kg、0.30g/kg、0.15g/kg)。以肌注苯甲酸雌二醇及黄体酮的方法复制大鼠乳腺增生模型,测量血清中激素水平(E2、LH、PRL、FSH、P),光镜下观察乳腺形态学变化及用免疫组化方法观察乳腺组织中雌激素受体(ER)和孕激素受体(PR)的变化。结果:与模型组比较,乳络通0.60g/kg、0.30g/kg剂量组能显著改善动物体内激素水平,使它们接近正常水平;乳络通0.60g/kg、0.30g/kg剂量组能够使大鼠乳腺增生模型乳腺组织中的ER、PR的阳性表达水平显著或者明显降低,显著抑制乳腺小叶增生。结论:乳络通通过调整激素水平,降低实验大鼠乳腺组织ER、PR含量,抑制乳腺小叶增生而起到抑制乳腺增生的作用。
Objective： To study the curative effects of Ruluotong capsules on mammary gland hyperplasia（ MGH） rats and provide the basis for the wider use. Methods： Wistar rats were randomly divided into six groups： the normal control group,the model group,the high,medium and low dose of Ruluotong groups（ 0. 60g /kg,0. 30g /kg,0. 15g /kg） and the Rukang Pill group（ 0. 53g /kg）. The back 5 groups were injected with benzestrofol and progesterone intramuscuarly to induce the MGH model,then given respective medicines via gastrogavage for 30days.The levels of E2,LH,PRL,FSH,P were measured The changes in morphology of mammary gland were observed using light microscope,and the expression of estradi-ol receptor（ ER） and progestogen receptor（ PR） were detected with immunohistochemistry. Results： Compared with the model group,the dosage of Ruluotong（ 0. 60g /kg,0. 30g /kg） after treatment,the levels of hormore were markedly adjusted（ P ＆lt; 0.01）,and the ER,PR positive expression was significantly decreased（ P ＆lt; 0. 01,P ＆lt; 0. 05）. Ruluotong can significantly reduce lobular hyperplasia of breast glands. Conclusions： Ruluotong can treat MGH by reducing the expression of ER and PR,lobular hyperplasia of breast glands.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第2期128-131,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
教育部优秀人才支持计划
课题编号NCET-04-0657
