摘要
F-box是Skp1-Cullin1-F-box(SCF)型泛素连接酶E3的重要组成部分,在泛素化介导的蛋白质降解中选择性识别靶蛋白。本文从谷子苗期干旱胁迫条件下构建的转录组文库中克隆了与耐旱早期响应相关的F-box基因,命名为SiFBX(GenBank登录号为KC252635.1)。该基因全长510 bp,编码170个氨基酸。蛋白质结构预测表明,该蛋白含有丰富的精氨酸、亮氨酸、丝氨酸,缺少跨膜结构域及信号肽序列。系统进化分析表明,该基因与已报道的EID1和FBW4亲缘关系较近。在该基因上游1.9 kb序列处,预测到启动子的核心序列及与多种逆境胁迫相关的调控序列。荧光定量PCR分析表明,该基因分别在正常干旱、PEG和ABA诱导下,表达量出现显著变化。
F-box proteins, components of the Skpl-Cullinl-F-box （SCF） protein E3 ubiquitin ligase complex, serve as the variablecomponent responsible for substrate recognition and recruitment in SCF-mediated proteolysis. The anti-drought relative gene ofSiFBX （GenBank accession number KC252635.1） which belongs to the F-box super family was cloned from foxtail millet （Se-taria italic）. The full-length cDNA of SiFBX was 510 bp, which encoded 170 amino acid residues. Protein analysis and structurepredication showed that it has a higher proportion of arginine （R）, leucine （L）, and serine （S） and a lack of trans-membrane do-mains and signal peptide. Phylogenetic analysis demonstrated that SiFBX has similarity with EID1 and FBW4. Many abioticstress-related cis-acting elements and transcription factors were discovered in the 1.9 kb upstream region of SiFBX. The results ofreal-time PCR showed that there were remarkable changes in the expectation level of SiFBX for the treatments with PEC~ wa-ter-withholding, and ABA.
出处
《作物学报》
CAS
CSCD
北大核心
2014年第6期1027-1034,共8页
Acta Agronomica Sinica
基金
辽宁省科技厅农业攻关项目(2011208001)资助
