摘要
目的研究艾氏腹水瘤全细胞瘤苗诱导的特异性杀伤活性。方法用40g/L的多聚甲醛处理的艾氏腹水瘤细胞作为全细胞瘤苗免疫Balb/c小鼠,建立瘤苗小鼠模型。用HE染色法对亲本肿瘤细胞皮下再次攻击形成的肿瘤结节进行病理学观察;用51Cr释放法测定瘤苗免疫组、荷瘤组、正常组小鼠脾细胞,对亲本艾氏腹水瘤细胞和Sp2细胞的杀伤活性。结果HE染色显示,经瘤苗免疫后亲本肿瘤细胞皮下再次攻击形成的肿瘤结节中,瘤细胞几乎完全坏死,同时在坏死部位有大量炎细胞浸润、纤维母细胞和小血管增生而对照组则无以上现象。效靶比为200∶1时,免疫小鼠脾细胞体外杀伤亲本艾氏腹水瘤细胞的杀伤率%为42.3±3.2%,显著高于荷瘤组的12.1±2.3%、正常组的6.1±1.1%和对Sp2/0细胞的杀伤率8.8±0.4%P均0.05。结论艾氏腹水瘤全细胞瘤苗可诱导机体产生特异性杀伤活性。
Aim To study the tumor specific cytotoxicity induced by Ehrlich ascites tumor cell vaccine. Methods Using paraformaldehyde treated Ehrlich ascites tumor cells as the vaccine, an animal model of tumor vaccination was established. The tumor nodules of the immunized Balb/c mice after subcutaneous tumor rechallenge were observed by HE staining. Splenocytes were separated from immunized, tumor bearing and control mice, respectively. The 51Cr release assay was used to examine the cytotoxicities of three kinds of the splenocytes to Ehrlich ascites tumor cells and Sp2/0 cells. Results It was showed that the tumor cells in tumor nodules of immunized mice exhibited almost complete necrosis and simultaneously possessed a large number of lymphocytes infiltration, fibroblasts and small vessels proliferation, while no phenomena mentioned above was found in the control mice. Specific cytotoxic rate of splenocytes from the immunized mice to the Ehrlich ascites tumor was (42.3±3.2)%,significantly higher than that from the other two groups(P∨0.05) and also significantly higher than that to Sp2/0 cells (8.8±0.4)%(P∨0.05). Conclusion Ehrlich ascites tumor cell vaccine can induce significantly tumor specific cytotoxicity in vivo.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第2期157-159,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助
No.39900136
