摘要
研究了基础饲料中添加不同浓度的黄芪多糖(astragalus polysaccharides,APS)对克氏原螯虾(Procambarus clarkii)抗白斑综合征病毒(white spot syndrome virus,WSSV)的效果。用添加0%、0.2%、0.4%、0.8%黄芪多糖的基础饲料作为药饵,投喂20 d后,对各组经PCR检测WSSV呈阴性的健康克氏原螯虾进行WSSV病毒悬液腹节背部注射攻毒试验,当添加0%黄芪多糖的阳性对照组中死亡率达到100%时,添加0.2%、0.4%、0.8%黄芪多糖实验组的死亡率分别为(86.67±13.33)%、(91.11±7.70)%、(73.33±17.64)%。为了评价黄芪多糖对克氏原螯虾各器官组织的影响,对其鳃、肝胰腺、心肌组织进行病理组织切片观察,结果发现:添加0%黄芪多糖的阳性对照组中克氏原螯虾的鳃、肝胰腺、心肌组织出现细胞排列无序、细胞破裂、核仁皱缩等明显的病理变化;而添加0.8%黄芪多糖的实验组中克氏原螯虾的鳃、肝胰腺组织未见明显病变,心肌组织核仁出现一定程度的皱缩,但细胞尚未破裂。研究表明:与阳性对照组相比,添加0.8%的黄芪多糖可提高26.67%的存活率,对克氏原螯虾抗WSSV感染有很好的提高效果,可望在生产中收到良好的经济效益。
We studied the effects of anti-infection of white spot syndrome virus（ WSSV） by adding different concentrations of astragalus polysaccharides（ APS） to the basal diet in Procambarus clarkii. The healthy P. clarkii which were negative of WSSV by PCR detection were injected intraperitoneally,with 100 μL WSSV, and fed with 0%,0. 2%,0. 4%,0. 8% of APS bait. After feeding 20 d,the result revealed that when the positive control group mortality rate was 100 ± 0%,the mortality of 0. 2%,0. 4%,0. 8 % APS addition groups were 86. 67% ± 13. 33%,91. 11% ± 7. 70%,73. 33% ± 17. 64%,respectively. In order to evaluate the effect of APS on visceral organs,such as gill,hepatopancreas,and myocardial tissue in P. clarkii,the tissue sections were observed. The results showed that there was significant pathological change like nuclear pyknosis,cell ruptured and arranged desultorily in the group of 0% APS bait,but there were no obviously pathological changes in the gills and hepatopancreas, whereas nucleoli displayed a certain degree of shrinkage,while the cells have not yet broken in the myocardial tissue in the group of adding 0. 8% APS bait. This experiment showed that the survival rate can be increased to 26. 67% when adding 0. 8% APS bait compared with the positive control in P. clarkii. The APS can significantly improve the effect of resistance of P. clarkii to the WSSV infection,and it can be expected to receive good economic benefits in the actual aquaculture production.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2014年第3期423-428,共6页
Journal of Shanghai Ocean University
基金
国家级大学生创新训练计划项目(B-5106-12-0002)
公益性行业(农业)科研专项(201003070)
上海高校知识服务平台(ZF1206)
关键词
黄芪多糖
白斑综合征病毒
克氏原螯虾
病理组织切片
astragalus polysaccharides
white spot syndrome virus （WSSV）
Procambarus clarkii
pathologictissue slice
