期刊文献+

产β-甘露聚糖酶菌株的物理和化学诱变育种研究 被引量:4

Physical and Chemical Mutagenesis Breeding of β-mannanase Strains
下载PDF
导出
摘要 [目的]比较产β-甘露聚糖菌株的物理和化学诱变育种效果。[方法]以产β-甘露聚糖酶的Bacillus subtilis WD23为出发菌株,利用紫外线诱变和甲基磺酸乙酯诱变的方法提高酶的活性。[结果]紫外线照射时间选为20~30s,诱变后酶活提高了8.72%;1%的甲基磺矗乙酯诱变10min,酶活提高了1.29%。[结论]紫外线诱变效果好于甲基磺酸乙酸,该研究中,物理诱变效果好于化学诱变。 [ Objective ] To compare physical and chemical mutagenesis breeding of 15-mannanase strains. [ Method]β-mannanase strains Bacillus subtilis WD23 was mutated by ultraviolet ray and ethylmethane sulfonate to increse its enzyme activity. [ Result] After mutated by UV irradiation for 20 -30 s or 1% thylmethane sulfonate for 10 min ,the enzyme activity of β-mannanase increase 8.72% and 1.29%, respectively. [ Conclusion ] The mutation effect by ultraviolet ray is better than ethylmethane sulfonate, and the physical mutation is better than chemical mutation.
出处 《安徽农业科学》 CAS 2014年第18期5741-5741,5750,共2页 Journal of Anhui Agricultural Sciences
基金 黑龙江中医药大学优秀创新人才支持计划(2012RCQ13)
关键词 Β-甘露聚糖酶 紫外线诱变 甲基磺酸乙酯诱变 β-mannanase Ultraviolet mutation Ethylmethane sulfonate mutation
  • 相关文献

参考文献7

  • 1MCCLEARY B V. β - mannanase [ J ]. Methods in Enzymology, 1988,160 : 596 -610.
  • 2SCHOMBUG D, SAIZMANN M. Enzyme Handbook [ M ]. Berlin : Springer - Verlay Berlin Heideberg, 1991 : 1 - 5.
  • 3COURTIOS J E,PETEK F,KADA T. Research on galactomannans. II. Action of takadiastase on the galaetomannan of lueeren [ J ]. Bull Soc Chem Bid, 1958,40:2031 - 2037.
  • 4WILLIAMS P P,DOEISCH R N. Microbial dissimilation of galactornannan [J]. Gen Microbio1,1960 ,22 :635 -644.
  • 5REESE T,SHIBATA Y. β-Mannanase of fungi[J]. Can J Microbiol,1965, 11:167 - 183.
  • 6MILLER G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar[J]. Anal Chem,1959(31 ) :426 -428.
  • 7庞晓坤,曹德菊,花日茂,张海云.物理因子诱变技术在废水生物处理中的应用研究[J].四川环境,2005,24(5):68-71. 被引量:8

二级参考文献19

共引文献7

同被引文献45

  • 1汪杏莉,李宗伟,陈林海,刘晓波,谢慧波.工业微生物物理诱变育种技术的新进展[J].生物技术通报,2007,23(2):114-118. 被引量:30
  • 2de SOUZA P M, MAGALHAES P O. Application of microbial a- amylase in industry--A review[J]. Brazilian Journal of Microbiol ogy,2010,41(4) :850-861.
  • 3van der MAAREL M J,van der VEEB B,UITDEHAAG J C,et al. Properties and applications of starch-converting enzymes of the t-amylase family[J]. J Biotechnol,2002,94(2) :137 -155.
  • 4FIEDUREK J ,GROMADA A. Screening and mutagenesis of mol- ds for improvement of the simultaneous production of catalase and glucose oxidase[J]. Enzyme and Microbial Technology, 1997,20 (5) ,344-347.
  • 5LI Tinglan, FAN Yuxiang, NAMBOU K, et al. Improvement of ansamitoein P-3 production by Actinosynnema mirum with fructose as the sole carbon source[J]. Applied Biochemistry and Biotechnology, 2015, 175(6): 2845-2856.
  • 6GAO Yang, FAN Yuxiaug, Nambou K, et al. Enhancement of ansamitocin P-3 production in Actinosynnema pretiosum by a synergistic effect of glycerol and glucose[J]. Journal of Industrial Microbiology & Biotechnology, 2014, 41(1): 143-152.
  • 7JURJENS G, KIRSCHNING A. Synthesis of a cytotoxic ansamycin hybrid[J]. Organic Letters, 2014, 16(11): 3000-3003.
  • 8SCHWENK D, NE'Iq" M, DAHSE H M, et al. Injury-induced biosynthesis of methyl-branched polyene pigments in a white- rotting basidiomycete[J]. Journal of Natural Products, 2014, 77 (12): 2658-2663.
  • 9NEWMAN D J, GIDDINGS L A. Natural products as leads to antitumor drugs[J]. Phytochemistry Reviews, 2014, 13(1): 123-137.
  • 10EICHNER S, KNOBLOCH T, FLOSS H G, et al. The interplay between mutasynthesis and semisynthesis: generation and evaluation of an ansamitocin library[J]. Angewandte Chemic Intemational Edition, 2012, 51(3): 752-757.

引证文献4

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部