摘要
目的研究海龙蛋白质提取物对宫颈癌Hela细胞增殖及凋亡的诱导作用。方法采用MTT法检测蛋白质提取物对细胞生长的抑制情况,采用Annexin V/PI流式细胞分析法检测细胞凋亡,Western blot检测凋亡蛋白Bax、Bcl-2、P53的表达。结果不同浓度海龙蛋白质提取物对Hela细胞的增殖有抑制作用,具有时间和浓度依赖性。Annexin V/PI流式细胞分析显示,加药组早期凋亡率分别为29.18±0.65、39.32±0.78、51.21±1.13、61.56±1.09,与对照组(9.82±0.18)差异有统计学意义(P<0.05);晚期凋亡率加药组分别为3.20±0.03、7.48±0.06、11.11±0.07、14.60±0.03,与对照组(1.12±0.09)差异有统计学意义(P<0.05)。Western blot检测发现随着蛋白质提取物浓度的升高,Bax、P53蛋白的表达逐渐升高,Bcl-2蛋白的表达逐渐降低。结论海龙蛋白质提取物在一定浓度范围内可在体外抑制Hela细胞增殖并诱导其凋亡,且随蛋白质提取液浓度的升高,凋亡率显著增加,可能的机制是增强促凋亡蛋白Bax、P53的表达,降低抗凋亡蛋白Bcl-2的表达。
Objective To study the role of Syngrathus protein extract in inducing proliferation and apoptosis of cervical cancer Hela cells.Methods The inhibitory effect of Syngrathus protein extract on growth of Hela cells was tested by MTT assay.Apoptosis of Hela cells was assayed by Annexin V/PI flow cytometry.Expression of Bax,p53 and Bcl-2 in Hela cells was detected by Western blot.Results Syngrathus protein extract inhibited the growth of Hela cells in a time-and concentration-dependent manner.Annexin V/PI flow cytometry showed that the early and late apoptosis rate of Hela cells were significantly higher in Syngrathus protein extract treatment group than in control group （29.18 ± 0.65,39.32 ± 0.78,51.21 ± 1.13,61.56 ± 1.09 vs 9.82 ± 0.18,P 〈 0.05; 3.20 ± 0.03,7.48 ± 0.06,11.11 ± 0.07,14.60 ± 0.03 vs 1.12 ± 0.09,P 〈 0.05）.Western blot displayed that the expression level of Bax and P53 protein increased while that of Bcl-2 protein decreased with the increasing concentration of Syngrathus protein extract.Conclusion Syngrathus protein extract inhibits the proliferation and induces the apoptosis of Hela cells in vitro by up-regulating the expression of Bax and P53 and down-regulating the expression of Bcl-2.The apoptosis rate of Hela cells increases with the increasing concentration of Syngrathus protein extract.
出处
《解放军医学院学报》
CAS
2014年第6期623-626,共4页
Academic Journal of Chinese PLA Medical School
