摘要
目的探讨人类表皮生长因子受体2（ErbB2）表达对人脑胶质瘤细胞侵袭能力的影响及其可能机制。方法在体外培养的胶质瘤TJ905细胞中分别转染ErbB2shRNA（ErbB2shRNA载体组）和ErbB2过表达载体（ErbB2过表达载体组），以下调或上调细胞中ErbB2表达水平，并以转染空载体质粒（ErbB2空载对照组1为对照。应用Western blotting检测ErbB2及基质金属蛋白酶-2（MMP-21、MMP-9蛋白的表达水平，应用Transwell小室侵袭实验评价各组细胞侵袭能力的变化。结果与ErbB2空载对照组ErbB2及MMP-2、MMP-9蛋白表达水平（62．34±5．72，62．34±5．72，69．76±6．25）相比．转染ErbB2shRNA载体后有效抑制了细胞中ErbB2及MMP-2、MMP-9蛋白表达（34．82±4．91，58．73±4．48，52．32±5．23），而转染ErbB2过表达载体后细胞中ErbB2及MMP-2、MMP-9蛋白表达水平明显升高（69．76±6．25，87．34±7．96，94．39±6．12），差异均有统计学意义（P〈0．05）。ERRB2shRNA载体组穿过上室Matrigel胶的平均细胞数（28．5个／视野）较ErbB2空载对照组（70个／视野1明显减少，而ERRB2过表达载体组（82个／视野）较ErbB2空载对照组明显增多。结论ErbB2表达水平变化可影响胶质瘤细胞的侵袭能力，其机制可能与MMP-2、MMP-9表达水平发生改变相关。
Objective To investigate the effect of epidermal growth factor receptor 2 （ErbB2） expression on invasion of glioma cells and its possible mechanism. Methods Glioma TJ905 cells were cultured in vitro; and ErbB2 shRNA and overexpression vectors were constructed and transfected into Glioma TJ905 cells to down-regulate and up-regulate the ErbB2 expression levels; empty vector plasmid was also transfected into the T J905 cells as control group. Invasive ability changes of T J905 ceils were measured by Transwell assay, and the expression levels of matrix metalloprotease （MMP）-2 and MMP-9 were identified by Western blotting. Results As compared with the ErbB2, MMP-2 and MMP-9 protein expression levels in the control group （62.34±5.72, 62.34±5.72 and 69.76±6.25）, those in the ErbB2 shRNA group were significantly decreased （34.82±4.91, 58.73±4.48 and 52.32±5.23）, while those in the ErbB2 overexpression group were significantly increased （69.76±6.25, 87.34±7.96 and 94.39±6.12）, with significant differences （P〈0.05）. The mean cells crossing Matrigel in the ErbB2 shRNA group （28.5 cells/field） were obviously decreased, and those in the ErbB2 overexpression group were increased （82 cells/field） as compared with those in the control group （70 cells/field）, with significant differences （P〈0.05）. Conclusion ErbB2 expression can affect the invasiveness of glioma cells, which might be related to the expression changes of MMP-2 and MMP-9.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2014年第6期586-589,共4页
Chinese Journal of Neuromedicine
基金
河南省自然科学基金（20118273）
