摘要
目的探讨肝细胞生长因子及其受体c-Met（HGF-cMet）的表达与结直肠癌同时性肝转移的关系。方法2001年6月至2010年6月间有30例结直肠癌同性性肝转移患者在山东省肿瘤医院接受原发癌和肝转移癌一期根治性切除术，根据其是否伴有区域淋巴结转移分为实验Ⅰ组（T1～T4N1～N2M1，21例）和实验Ⅱ组（T1-T4N0M1，9例）。并选取与实验I组匹配的T4-T4N1-N2M0患者（21例）和T1～T4N0M0患者（21例）以及与实验Ⅱ组匹配的T1-T4N0M0患者（9例）作为对照。应用免疫组织化学方法检测原发灶、淋巴结转移灶及其肝转移灶中HGF和c-Met的蛋白表达水平。结果实验Ⅰ组（T1～T4N1-N2M1）原发灶HGF阳性表达率[71％（15／21）]明显高于其匹配的T1～T4N1～N2M0对照组[43％（9／21）]和T1～T4N0M0对照组[19％（4／21）]（均P〈0．05）；且c—Met阳性表达率[90％（19／21）]也明显高于T1～T4N0M0对照组[43％（9／21），P〈0．05]，但与T1-T4N1-N2M0对照组[86％（18／21）]的差异则无统计学意义（P〉0．05）。实验Ⅱ组（T1～T4N0M1）与其对照组（T1～T4N0M0）原发灶HGF阳性表达率（6／9比5／9．P〉0．05）和c-Met阳性表达率（8／9比6／9，P〉0．05）的差异均无统计学意义。实验Ⅰ组患者原发灶、淋巴结转移灶及肝转移灶中HGF和c—Met表达一致率分别为81％（17／21）和76％（16／21）。结论HGF及其受体c—Met可能对存在区域淋巴结转移的结直肠癌同时性肝转移的发生具有一定作用，而对无淋巴结转移的结直肠癌发生肝转移的作用可能较小。
Objective To investigate the association between expression of hepatocyte growth factor （HGF） and its receptor c-Met and primary colorectal cancers with synchronous liver metastases. Methods A total of 30 colorectal cancer patients with synchronous liver metastasis underwent radical resection of primary cancer and liver cancer in our hospital from June 2001 to June 2010. According to lymphatic metastasis, patients were divided into group A（T1-T4N 1-N2M 1, n=21） and group B（T1-T4N0M1, n=9）. Twenty-one matched T1-T4N1-N2M0 and 21 T1-T4N0M0 patients were used as the controls of group A. Nine matched T1-T4N0M0 patients were used as the controls of group B. Expressions of HGF and c-Met in tissues of primary loci, liver loci and metastatic loci were detected by immunohistochemistry. Results In primary loci of group A, the positive rate of HGF was significantly higher than that of T1 -T4N1 -N2M0 and T1-T4N0M0 controls [71%（15/21） vs. 43%（9/21）, 19%（4/21）, all P〈0.05]. The positive rate of c-MET [90%（19/21）] was significantly higher compared to T1-T4N0M0 control [43%（9/21）, P〈0.05], while not significantly different compared to T1 -T4N1 -N2M0 control [86% （18/21）1. In primary loci of group B, positive rates of HGF and c-MET were not significantly different as compared to T1 -T4N0M0 control[6/9 vs. 5/9, PM）.05; 8/9 vs. 6/9, P〉0.05]. Concordance of HGF and c-MET expression in group A among primary loci, lymphatic metastatic loci and hepatic metastatic loci was 81%（17/21） and 76% （16/21）. Conclusion HGF-c-Met may play a role in colorectal cancer patients with synchronous liver metastasis who have regional lymphatic metastasis, and may have few effect on colorectal cancer with synchronous liver metastasis without corresponding lymphatic metastasis.
出处
《中华胃肠外科杂志》
CAS
CSCD
2014年第6期598-601,共4页
Chinese Journal of Gastrointestinal Surgery
基金
山东省科技发展计划项目（2010g0021859）
山东省自然科学基金（ZR2010HL024）
