摘要
目的:研究卵巢癌细胞、卵巢癌腹水、人外周血树突状细胞、人脐带血树突状细胞4种来源外泌体对人外周血来源巨噬细胞功能的影响。方法:采用超速离心过滤法分离取得4种来源外泌体、细胞因子刺激法获得外周血来源的巨噬细胞,观察4种外泌体对巨噬细胞一氧化氮产生、杀瘤活性及吞噬功能的作用。结果:卵巢腺癌细胞SK-OV-3分泌的外泌体和卵巢癌腹水分泌的外泌体均降低了巨噬细胞分泌一氧化氮水平、吞噬功能及杀瘤活性,人外周血树突状细胞和新生儿脐带血树突状细胞分泌的外泌体均提高了巨噬细胞分泌一氧化氮水平、吞噬功能及杀瘤活性;卵巢癌外泌体组杀瘤活性与对照组差异无统计学意义(P>0.05),其余与对照组相比差异均有统计学意义(P<0.05)。结论:肿瘤细胞及腹水来源外泌体对巨噬细胞功能具有抑制作用,外周血和脐血树突状细胞来源外泌体对巨噬细胞功能具有促进作用。
Objective To research the effects of exosomes from ovarian cancer cells, ascites of ovarian cancer, human peripheralblood dendritic ceils and human umbilical cord blood dendritic cells on the function of macrophage in peripheral blood. Methods： Uhracen-trifugation and uhrafihration method was used to obtain exosomes of four different resources ; cytokine stimulation method was used to obtainmacrophages in peripheral blood ; the effects of exosomes of four different resources on nitric oxide production, phagocytosis and tumor killingactivity of macrophages were observed. Results： Exosomes secreted by ovarian adenocarcinoma SK - OV - 3 cells and ascites of ovariancancer reduced nitric oxide productien, phagocytosis and tumor killing activity of macrophages ; exosomes secreted by human peripheral blooddendritic cells and human umbilical cord blood dendritic cells improved nitric oxide production, phagocytosis and tumor killing activity ofmacrophages; there was no statistically significant difference in tumor killing activity of maerophages between ovarian cancer group and control group （ P 〉 0. 05 ）, there were statistically significant differences between the other groups and control group （ P 〈 0. 05 ） . Conclusion：Exosomes from ovarian cancer ceils and ascites of ovarian cancer can inhibit function of macrophages, exosomes from human peripheral blooddendritic cells and human umbilical cord blood dendritic cells promote function of macrophages.
出处
《中国妇幼保健》
CAS
北大核心
2014年第18期2973-2975,共3页
Maternal and Child Health Care of China
基金
吉林省科技厅自然科学基金资助项目〔201015232〕
吉林省卫生厅课题资助项目〔2011Z035〕
关键词
卵巢癌
树突状细胞
外泌体
Ovarian cancer
Dendritic cells
Exosome
