摘要
探讨了超声波辅助提取大叶黄杨中总黄酮的最佳提取工艺及其总黄酮的抗氧化能力。采用单因素和正交试验确立了大叶黄杨中总黄酮的最佳提取工艺。结果表明,超声波辅助法提取大叶黄杨叶中总黄酮的最佳工艺为:乙醇浓度80%、料液比1∶30(g/mL)、超声提取时间60min、提取2次,并对最佳工艺进行验证,验证实验表明总黄酮提取率平均为3.82%;通过大叶黄杨中总黄酮对·OH、O-2·的清除能力和还原能力测定,结果表明:与VC相比,对·OH的清除能力更强,而对O-2·清除能力和还原能力较弱,表明大叶黄杨的总黄酮具有一定的抗氧化能力。本研究能为大叶黄杨作为药用植物的开发利用提供理论支持。
Ultrasonic-assisted extraction of flavonoids from Euonymus japonicus and their antioxidant activities werefirst investigated in this study.The optimum extraction condition for fiavonoids was established by single-factor andorthogonal experiment.The optimal extraction conditions for flavonoids were as follows-solid to liquid ratio for 1：30（g/mL）, 80% ethanol concentration, ultrasonic extraction for 60min and extraction twice. And this optimalextraction condition of orthogonal experiment was verified ,the average fiavonoids extraction yield was 3.82%.Also,scavenging activities of the flavonoids on hydroxyl, superoxide anion and reducing power were determined,theresults showed that compared with positive control Vc ,flavonoids from Euonymus japonicus excelled in scavengingactivity on hydroxyl radical,whereas it had weaker activity on scavenging superoxide anion radical as well as thereducing power. These results clearly indicated that flavonoids from Euonymus japonicus were effective inscavenging free radicals and had the potential to be antioxidants and also provided the theoretical data forsupporting the use of Euonymus japonicus as medicinal plant.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第13期266-270,共5页
Science and Technology of Food Industry
基金
河南城建学院博士启动基金资助
关键词
超声波辅助提取
大叶黄杨叶
工艺条件
总黄酮
抗氧化能力
ultrasonic- assisted extraction
Euonymus japonicus
process condition
flavonoids
antioxidant activity
