摘要
目的：研究药对巴戟天-杜仲总多糖对体外培养的大鼠软骨细胞增殖活性的影响。方法：①用水提醇沉法、Sevag法除蛋白提取纯化获得其总多糖，苯酚-硫酸比色法测定其总多糖含量；②MTT法分别检测体外培养大鼠软骨细胞在800，400，200，100，50μg·mL-1总多糖DMEM溶液中24，48，72 h的增殖能力。结果：①葡萄糖标准品线性回归方程为y=0.0100x-0.0018（r=0.9996），葡萄糖标准品在10～100μg·mL-1范围内线性关系良好，平均加样回收率为99.59%，RSD为0.40%；多糖含量为43.88%。②巴戟天-杜仲总多糖对软骨细胞具有一定的促增殖作用，当作用时间为48 h、多糖浓度为400μg·mL-1时，促增殖作用最强。结论：①苯酚-硫酸比色法可作为药对巴戟天-杜仲总多糖的含量测定方法；②巴戟天-杜仲总多糖对软骨细胞的促增殖作用具有一定的时效量效关系。
Objective：To study the effects of polysaccharide in couplet drugs of Bajitian（Radix Morindae Officinalis）and Duzhong（Cortex Eucommiae）on the proliferation of rat chondrocytes cultured in vitro.Methods： ① The total polysaccharide was extracted and purified by the water extraction and alcohol pre-cipitation and the Sevag method,measuring its content by Phenol-sulfuric acid colorimetric method; ② By the MTT method,the reproductive activity of rat chondrocytes was detected in the polysaccharide DMEM solution of 800,400,200,100,50 μg·mL-1 for 24,48,72 hs.Results： ① The standard glucose linear regression equation was y=0.010 0x-0.001 8 （r=0.999 6）.The linear relationship of standard glucose was good in 10~100 μg·mL-1, the average recovery being 99.59%,RSD being 0.40%and the content of polysaccharide being 43.88%. ② The total polysaccharides of Bajitian（Radix Morindae Ofifcinalis）and Duzhong（Cortex Eucommiae）could pro-mote the reproductive activity of rat chondrocytes.The promoting function was strongest when the concentration of polysaccharide was 400 μg·mL-1 for 48 hs.Conclusion：①The Phenol-sulfuric acid colorimetric method can be used to measure the content of polysaccharide in couplet drugs of Bajitian（Radix Morindae Ofifcinalis）and Duzhong（Cortex Eucommiae）;②The function of polysaccharide in promoting the reproductive activity of rat chondrocytes is related to a certain time,quantity and effectiveness.
出处
《风湿病与关节炎》
2014年第6期23-26,共4页
Rheumatism and Arthritis
基金
福建省自然科学基金项目(13141220)
关键词
软骨细胞
增殖
药对巴戟天-杜仲
多糖
含量测定
大鼠
cartilage cell
proliferation
couplet drugs of Bajitian （ Radix Morindae Officinalis ） and Duzhong （ Cortex Eucommiae ）
polysaccharide
content measurement
rats
