摘要
以体积分数50％的甲醇溶液为溶剂，用超声提取法从开口箭药材中提取绿原酸，用高效液相色谱法检测提取液中绿原酸含量。提取条件为：超声功率240 W，超声频率40 kHz，提取温度60℃，超声提取30 min。色谱条件为：色谱柱为Inertsil ODS-3C18（4．6 mm ×150 mm，5μm）柱，以乙腈-0．4％磷酸水溶液（体积比9∶91）为流动相，流速1．0 mL/min，检测波长327 nm，柱温30℃。绿原酸在0．255～3．06μg/mL范围内与峰面积呈现良好的线性关系， y ＝31388x＋152．43（R^2＝0．9999），平均加样回收率99．4％，RSD为2．12％（n＝3）。检测结果表明，不同产地开口箭药材中绿原酸的含量有一定的差异，其变化范围在32．84～6．62μg/g。该方法简便、准确、重复性好，可用于开口箭药材的质量控制。
The chlorogenic acid is extracted from Tupistra chinensis using 50%methanol , and the conditions of the ultrasonic wave are as follows： extraction ultrasonic power of 240 W, ultrasonic frequency of 40 kHz,temperature of 60 ℃ and ultrasonic treatment time of 30 min.Samples are analyzed on an Inertsil ODS-3C18 column（4.6 mm ×150 mm, 5 μm） using acetonitrile-0.4%phosphoric acid as mobile phase , and speed of flow is 1.0 mL/min, the examination wave length is 327 nm and the column temperature is 30 ℃. The chlorogenic acid shows a good linearity in the range of 0.255-3.06 μg/mL, egression equation being y =31 388x ＋152.43（R^2 =0.999 9） and the average recovery being 99.4%（RSD=2.12%）.The result shows that there are some differences in the content of chlorogenic acid in Tupistra chinensis among different origins .The established method is simple , credible , accurate , repeatable and can be used for the quality control of Tupistra chinensis.
出处
《陕西理工学院学报（自然科学版）》
2014年第3期59-62,73,共5页
Journal of Shananxi University of Technology:Natural Science Edition
基金
陕西省教育厅重点实验室科学研究计划项目(14JS)
陕西理工学院研究生创新基金资助项目(SLGYCX1312)
关键词
开口箭
绿原酸
含量测定
HPLC
Tupistra chinensis
chlorogenic acid
HPLC
determination of the content
