摘要
目的观察三维适形调强放疗(IMRT)对大鼠骨细粒棘球蚴原头节的杀灭效果,探讨IMRT对骨细粒棘球蚴病的治疗有效性。方法选取双股骨骨细粒棘球蚴病大鼠30只,对30只大鼠左侧骨细粒棘球蚴病区给予总剂量为40Gy的IMRT放疗,于放疗结束3d后,取鼠左、右侧股骨细粒棘球蚴原头节,行伊红染色,检测原头节死亡率;采用酶化学染色法检测原头节琥珀酸脱氢酶(SDH)的活性变化,观察IMRT对大鼠骨细粒棘球蚴的治疗效果。结果 IMRT作用3d后,原头节伊红染色结果可观察到,经IMRT治疗的大鼠左侧股骨的骨细粒棘球蚴原头节整体结构消失,出现深蓝色着色,原头节死亡率为(51.22±19.34)%,远高于未经IMRT治疗的右侧骨细粒棘球蚴原头节死亡率(3.55±1.97)%,差异有统计学意义(P<0.05);原头节的琥珀酸脱氢酶(SDH)检测发现,经IMRT治疗的大鼠左侧股骨的骨细粒棘球蚴原头节的琥珀酸脱氢酶活性较右侧明显减弱,且左侧骨细粒棘球蚴原头节琥珀酸脱氢酶SDH产物的平均光密度数值为(0.719 3±0.114 5),远大于右侧的(0.101 5±0.032 0),差异有统计学意义(P<0.05)。结论 IMRT可促进鼠骨细粒棘球蚴原头节的死亡,其作用机制可能是通过改变琥珀酸脱氢酶的活性而实现的。
Objective To observe the possibility of the killing effect of intensity modulated radiation therapy （IMRT） to the protoscolex of bone echinococcosis in rats, and explore the killing effectiveness of IMRT to the protoscolex of bone echinococcosis in rats. Methods 30 purebred bone echinococcosis rats were selected and the left thighbone of the rats were irradiated by the models of intensity modulated radiotherapy, the total irradiation doses were 40 Gy. The left and right samples of protoscolex of bone echinococcosis were taken out from the bone on one week after radiation. All the samples were observed by the Hematoxylin-Eosin staining and Succinate dehydrogenase. Results Three days later, the IMRT treatment of rats on the left side of the femur bone fine grained spines ball larva had obvious radiation to kill the original head quarter, and didnot appear on the right side without IMRT treatment; The head section of succinate dehydrogenase （SDH）, found that the rats on the left side of IMRT treatment of femoral bone fine grained spines ball larva original section head of succinate dehydrogenase activity is on the right side decreased significantly. Conclusion It is evident that the IMRT can promote rat bone fine grained spines ball larva original section head of death, its mechanism may be through changing the activity of succinate dehydrogenase.
出处
《新疆医科大学学报》
CAS
2014年第7期817-820,共4页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区科技支疆项目(2013911129)
新疆维吾尔自治区青年科技创新人才培养工程项目-青年博士科技人才培养项目(2013731013)
国家自然科学基金(81360276)
新疆维吾尔自治区自然科学基金(2012211B34)
中国博士后科学基金(2014M552566XB)
关键词
三维适形调强放疗
包虫
骨
原头节
intensity modulated radiation therapy
echinococcosis
bone
protoscolex
