摘要
本试验以表达猪乳铁蛋白的重组乳酸乳球菌饲喂断奶仔猪,研究其作为生态友好安全型的饲料添加剂替代抗生素的可行性。试验选取体重相近的36头断奶仔猪,分为3组,每组3个重复,每个重复4头。对照组饲喂基础饲粮,抗生素组饲喂添加20 mg/kg黄霉素和110 mg/kg金霉素的基础饲粮,试验组在饲喂基础饲粮的基础上每日按仔猪体重添加1次表达猪乳铁蛋白的重组乳酸乳球菌发酵菌液,每头仔猪每天饲喂乳铁蛋白含量约为50μg/kg。试验期21 d。结果表明:在饲粮中添加表达猪乳铁蛋白的重组乳酸乳球菌,与对照组相比,平均日增重显著提高(P<0.05),料重比极显著降低(P<0.01);与对照组和抗生素组相比,胸腺指数和脾脏指数均显著提高(P<0.05),盲肠中大肠杆菌数量极显著降低(P<0.01),盲肠中乳酸乳球菌数量极显著提高(P<0.01),十二指肠、空肠的绒毛高度/隐窝深度极显著提高(P<0.01);与对照组相比,血清中免疫球蛋白G、肠黏液中分泌型免疫球蛋白A含量显著提高(P<0.05),与抗生素组相比未达到显著水平(P>0.05);血清中白细胞介素-1含量显著高于对照组和抗生素组(P<0.05),血清中白细胞介素-2含量极显著高于对照组(P<0.01),显著高于抗生素组(P<0.05)。由此得出,在断奶仔猪饲粮中添加表达猪乳铁蛋白的重组乳酸乳球菌可促进仔猪肠道黏膜绒毛的发育,提高仔猪的饲料利用率,降低料重比,同时增强仔猪免疫功能,具有提高仔猪生长性能的作用。
This experiment was conducted to investigate the feasibility of recombinant Lactococcus lactis expressing porcine lactoferrin as an ecological friendly secure additive to substitute antibiotics feeding weaner piglets. A total of 36 weaner piglets with similar body weight were selected and randomly divided into 3 groups with 3 replicates in each group and 4 piglets in each replicate. The control group was fed a basal diet, the antibiotics group was fed the basal diet added 20 mg/kg flavomycin and 110 mg/kg chlortetracycline, while the trial group was fed the basal diet added recombinant Lactococcus lactis fermentation liquid （supplement according to the body weight of piglets, and the contain of porcine lactoferrin was 50 μg/kg per piglet per day） once a day. The experiment lasted for 21 days. The results showed that adding the recombinant Lactococcus lactis expressing porcine lactoferrin in the piglet diet, average daily gain was significantly higher than that in control group （P0.05）; the content of interleukin-1 in serum was significantly higher than that in control group and antibiotics group （P〈0.05）, the content of interleukin-2 in serum was significantly higher than that in control group （P〈0.01）, and significantly higher than that in antibiotics group （P〈0.05）. Adding recombinant Lactococcus lactis expressing porcine lactoferrin in weaner piglet diets can promote the growth of piglet intestinal mucosal villus, improve piglet feed utilization rate, decrease the feed/gain, at the same time, strengthen the piglet immune function, and has the effect of improving piglet growth performance.
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第6期1579-1586,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(30172057
30178709)
黑龙江省高校科技创新团队项目(2011TD001)
关键词
猪乳铁蛋白
乳酸乳球菌
断奶仔猪
替代抗生素
porcine lactoferrin
Lactococcus lactis
weaner piglets
instead of antibiotics
