摘要
测定干酪乳杆菌(Lactobacilluscasei)LC2W和植物乳杆菌(Lactobacillusplantarum)ST-III在以非消化性寡糖为唯一碳源的MRS液体培养液中的吸光值,以及在添加非消化性寡糖的脱脂牛乳中发酵后,发酵乳中益生菌菌落总数、乳酸含量和pH,研究非消化性寡糖对L.caseiLC2W和L.plantarumST-III体外增殖的影响。结果显示,低聚半乳糖和菊粉可以明显促进L.caseiLC2W的体外增殖(P<0.01),其最佳添加浓度为3%;而对L.plantarumST-III体外增殖促进作用最显著的是棉子糖和菊粉(P<0.01),最佳添加浓度为2%。研究表明不同益生菌菌株对非消化性寡糖发挥体外增殖效应具有选择性,研究结果可为益生菌产品的开发提供技术基础。
In order to evaluate the effects of nondigestible oligosaccharides（NDOs） on the in vitro growth of two probiotic strains,L.casei LC2W and L.plantarum ST-III,the absorption values of MRS cultures at 600 nm with NDOs as the unique carbon source were measured,and the colony count,lactic acid content,and pH value of fermented skim milk with the addition of NDOs were detected in this paper.The results showed that galactooligosaccharide（GOS） and inulin could promote the proliferation of L.casei LC2W significantly（P0.01）,and the optimal content of GOS and inulin was 3 %.Raffinose and inulin had a most remarkable influence（P0.01） on the growth of L.plantarum ST-III,and the optimal concentration of additive was 2 %.The research indicated that NDOs have selectivity on the in vitro proliferation of probiotic strains.The results can provide technical basis of the research and development of probiotic products.
出处
《食品研究与开发》
CAS
北大核心
2014年第8期97-101,共5页
Food Research and Development
基金
国家973计划项目(2010CB735705)
关键词
非消化性寡糖
干酪乳杆菌
植物乳杆菌
体外增殖
nondigestible oligosaccharides
Lactobacillus casei
Lactobacillus plantarum
proliferation in vitro
