摘要
为了培养本科生的分子生物学实验设计与操作技能,在实践教学中实现培养本科创新人才的目标,以用大肠杆菌发酵生产重组细菌碱性磷酸酶为案例,通过碱性磷酸酶基因的克隆、原核表达、发酵生产、提取纯化以及酶活性检测等系列实验,把本科的基因工程、发酵工程和生物化学3门综合性独立实验课程有机地组合成一个内容相关联的超大型综合性生物技术大实验,进一步凸显了生物技术中以基因工程技术为核心的上游核酸操作、中游发酵生产和下游蛋白分离纯化三大技术模块的有机联系,大大地提高了本科实验教学的综合性和研究性,提升了实践教学水平,取得了良好的教学效果。
In order to introduce a complete technique of biotechnology into an undergraduate experimental course,the bacterial alkaline phosphatase gene was chosen as an example to be cloned and over-expressed in Escherichia coli cells. The alkaline phosphatase was expected to be produced in large scale through fermentation,and the subsequent product could be purified and analyzed by biochemical methods. The design organically integrated our original three individual experimental courses-gene engineering,microbial fermentation and biochemistry as a super comprehensive course of biotechnology. The course included three tightly-related,but also relatively separated substantial domains-an up-stream module for alkaline phosphatase gene manipulation,a middle-stream module for microbial fermentation of alkaline phosphatase,and a down-stream module for biochemistry of alkaline phosphatase. Here,the paper briefly reviewed the break-through in biotechnology and described the contents and practical arrangement of each module in a proper semester,establishing the necessity to set such a comprehensive course in training creative talents of biotechnology for the future.
出处
《生物学杂志》
CAS
CSCD
2014年第3期104-107,共4页
Journal of Biology
基金
西部高校综合实力提升计划项目内蒙古大学生物化学系列课程教学卓越团队
关键词
实验教学
生物技术
基因工程
experimental teaching
biotechnology
genetic engineering
