摘要
以控制拟南芥春化途径的关键基因FLC全长CDS为参考序列,在萝卜属（Raphanus）EST数据库中进行BLASTN检索,得到具备Score≥100、E-value≤10^-10和coverage≥70%条件的70条萝卜ESTs作为候选的拟南芥FLC基因的同源ESTs,并进行序列聚类拼接、注释及分析。结果显示：在普通萝卜和野生萝卜中各发现两个旁系同源基因（RsFLCa和RsFLCb及RrFLCa和RrFLCb）。RFLCs与拟南芥FLC间在核酸水平上的一致性为78.7%～86.9%;RFLCs旁系同源基因间的一致性为75.9%～93.4%;RFLCs与芸薹属FLC直系同源基因间的一致性绝大多数维持在85%以上,且高于与拟南芥直系同源基因间的一致性。系统进化分析发现,与白菜、甘蓝及甘蓝型油菜中FLC多拷贝产生的机制可能类似,萝卜属FLC基因的多拷贝现象可能也是伴随萝卜基因组的多倍化而产生的。
FLC（ FLOWERING LOCUS C） in Raphanus species were identified using data mining method from Raphanus EST database. The full length CDS sequence of Arabidopsis thaliana vernalization pathway key gene FLC was used as reference to search the homologous ESTs in Raphanus EST database. Sequences with Score ≥100,Evalue ≤10^-10and coverage ≥70% were considered as the candidate FLC-gene-like ESTs,which were assembled and annotated in the following analysis. The results showed that 70 FLC-gene-like ESTs were identified in Raphanus,and four FLC candidate genes（ RsFLCa,RsFLCb,RrFLCa and RrFLCb） were predicted,among which RsFLCa and RsFLCb were originated from Raphanus sativus,and the other two were originated from Raphanus raphanistrum. The homologous FLC genes in Raphanus and Arabidopsis thaliana shared 78. 7%-86. 9% nucleotide sequence identity,and paralogs FLC genes in Raphanus shared 75. 9%-93. 4% nucleotide sequence identity. Identities of FLC orthologs between Raphanus and Brassica were more than 85%,which was higher than that between Raphanus and A. thaliana. Moreover, phylogenetic analysis suggested that multi-copy of FLC genes in Raphanus may be corresponded with genome polyploidy of Raphanus spieces,which was similar to that of FLC genes in Brassica species.
出处
《浙江农业学报》
CSCD
北大核心
2014年第3期656-660,共5页
Acta Agriculturae Zhejiangensis
基金
江苏省科技支撑计划项目(BE2012342)
江苏省农业科技自主创新资金项目[CX(12)2006]
盐城市农业科技发展计划项目(YC201003)
