摘要
利用基于原子力显微镜(AFM)的力谱技术,在正常生长的单个活细胞表面上,实时动态地研究了免疫毒素LHRH-PE40对HeLa细胞表面硬度的影响。采用Hertz-Sneddon模型计算所得力曲线相应的杨氏模量。实验表明,LHRH-PE40会引发HeLa细胞表面硬度逐步增加,且这种硬度的增加与细胞内微丝骨架的重组聚集有关系。本研究为全面掌握LHRH-PE40的药用效果和作用机理提供了重要信息。
The quantitative analysis of biomechanical profiles at the single-cell level can provide additional information. It is usually not available in traditional cell biology approaches,but may be crucial to assess and understand tumor prognosis and response to chemotherapy. In this study,the on-line changes of cell elastic properties after the addition of cancer target drug LHRH-PE40 were monitored by atomic force microscopy（AFM） on living HeLa cell surface under physiological condition. The results from AFM based force spectroscopy showed that LHRH-PE40 induced a distinct increase of the cell surface elasticity of HeLa cells. The fluorescence images implied that the target drug LHRH-PE40 would affect the re-organization of cell actions,which led to the increase of the elasticity of HeLa cells.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2014年第6期811-816,共6页
Chinese Journal of Analytical Chemistry
基金
国家重点基础研究发展计划(973计划)(No.2011CB935800)
国家自然科学基金(Nos.20975096
21075121
21275140和21375122)资助~~
关键词
细胞硬度
单细胞
力谱
原子力显微镜
Cell elasticity
Single-cell level
Force spectroscopy
Atomic force microscopy
