摘要
目的：优选制何首乌的β-环糊精（β-CD）辅助提取工艺.方法：采用层次分析法,以二苯乙烯苷、大黄素及大黄素甲醚转移率的综合评分为指标,通过正交试验考察β-CD用量、料液比、提取次数及时间对制首乌有效成分提取工艺的影响.采用HPLC测定二苯乙烯苷、大黄素及大黄素甲醚含量,流动相乙腈（A）-0.1％磷酸水（B）梯度洗脱（0～5 min,15％～25％ A;5 ～ 12 min,25％ ～30％ A;12～15 min,30％ ～60％ A;15 ～20 min,60％ A;20 ～25 min,60％ ～75％ A;25 ～ 31 min,75％ ～77％A）,检测波长320（二苯乙烯苷）,254 nm（大黄素、大黄素甲醚）.结果：最佳工艺条件为β-CD加入量10％,加10倍量水提取3次,每次1h;二苯乙烯苷、大黄素及大黄素甲醚转移率分别为90.19％,8.39％,0.76％.结论：与传统水提法相比,β-CD辅助提取法能显著提高活性成分转移率.
Objective： To optimize β-cyclodextrin （β-CD） assisted extraction process of Polygoni Multiflori Radix Praeparata. Method： Analytic hierarchy process was adopted, taking comprehensive score of transfer rates of emodin, physcion and stilbene glycoside as index, orthogonal test was applied to optimize extraction technology with solid-liquid ratio, β-CD dosage, extracting time and times as factors. HPLC was employed to determine contents of emodin, physcion and stilbene glycoside with mobile phase of acetonitrile （A） -0. 1% phosphoric acid solution （B） for gradient elution （0-5 min, 15%-25% A; 5-12 min, 25%-30% A; 12-15 min, 30%-60%A; 15-20 min, 60%A; 20-25 min, 60%-75%A; 25-31 min, 75%-77%A） and detection wavelength at 320 （stilbene glycoside） and 254 （emodin and physcion） nm. Result： Optimal extraction process was as following： extracted 3 times with 10-fold water for 1 h each time, β-CD dosage of 10% ; Transfer rates of emodin, physcion and stilbene glycoside were 8.39% , 0.76% and 90. 19% , respectively. Conclusion： Compared with traditional water extraction, β-CD assisted extraction process could significantly improve transfer rate of active ingredients.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第13期18-21,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
高等学校博士学科点专项科研基金项目(20111210110010
20111210120006)
关键词
Β-环糊精
制首乌
层次分析法
二苯乙烯苷
大黄素
大黄素甲醚
β-cyclodextrin
Polygoni Multiflori Radix Praeparata
analytic hierarchy process
stilbeneglycoside
emodin
physcion
