摘要
以西葫芦DH12-11409双单倍体植株自交后代种子为试材,研究不同激素组合、外植体类型和苗龄对诱导西葫芦不定芽再生以及不定芽生根的影响。结果表明,取5d龄西葫芦双单倍体黄绿色子叶的子叶节为外植体,在MS＋30g/L Suc＋8g/L Agar＋2.0mg/L 6-BA＋0.1mg/L NAA培养基上诱导不定芽效果最好,诱导率高达90.0%,分化系数为8.5;生根培养基则以MS培养基中添加0.05或0.1mg/L NAA为宜;5-6片真叶再生植株经驯化移栽成活率高达90.0%,为再生植株驯化移栽的最佳时期。
Taking the double haploid selfing generation seeds of Cucurbita pepo L.DH12-11409as test materials,we researched on induction effect of different combinations of hormone,explant types and seedling age on adventitious buds and shoot rooting.Studies showed that explants of 5-day-aged yellow-green cotyledon node from double haploid Cucurbita pepo L.grown on MS＋30g/L Suc＋8g/L Agar＋2.0mg/L 6-BA ＋0.1mg/L NAA medium had the best effect on adventitious buds induction with the induction rate of 90.0%and differentiation factor of 8.5.MS medium supplemented with0.05or 0.1mg/L NAA was appropriate for adventitious buds rooting.The best period for domesticated transplanting was regenerated plants with 5-6leaves and the survival rate was up to 90.0%.
出处
《西北农业学报》
CAS
CSCD
北大核心
2014年第6期134-140,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家大宗蔬菜产业技术体系项目(CARS-25)
公益性行业(农业)科研专项(201303112)
关键词
西葫芦
双单倍体
不定芽
植株再生
Cucurbita pepo L.
Double haploid
Adventitious buds
Plant regeneration
