摘要
用RT PCR方法对 2例感染新型HEV的样品进行了检测 ,并对PCR产物进行了克隆及测序。检测结果显示用常规PCR检测易造成ORF3中GC丰富区的缺失 ,但在常规PCR反应液中加入GCmelt溶液可成功地扩增GC丰富区。序列分析显示T1和T11属于同一基因型 ,但不同于已报道的HEVI型、II型和III型 ,为一新的基因型。T1和T11与I型在该区的核苷酸同源性为 79%～ 82 % ;与II型的同源性为 80 %～ 81% ;和III型的同源性为 83%～ 85 %
ORF3 and partial ORF1 regions were amplified with RT PCR from two patients (T1 and T11)infected with new genotype of hepatitis E Virus. The PCR products were cloned and sequenced. The results showed that G C rich region in ORF3 was deleted when amplified with normal PCR reaction. However, PCR reaction containing G C melt solution can overcome this problem. The sequence analysis showed that T1 and T11 belong to a new genotype of HEV which differs from genotype I,II and III reported.T1 and T11 have 79%～82%, 80%～81% and 83%～85% identical to genotype I,II and III respectively.
出处
《中国病毒学》
CSCD
2001年第1期28-33,共6页
Virologica Sinica
基金
英国WellcomeTrust基金组织资助
