摘要
轮状病毒是世界范围内引起婴幼儿腹泻的主要病原。根据病毒外壳蛋白VP4和VP7抗原性的不同可区分为不同型 :P(VP4,proteasesensitive)型和G(VP7,glycoprotein)型。 1998～ 1999年在中国 8个城市 (长春、秦皇岛、北京、杭州、福州、广州、成都、昆明 )采集了急性腹泻患儿的 10 93份非细菌性腹泻粪便标本 ,先进行A组轮状病毒(HRV)的筛选 ,其中阳性标本 433份 (39 6 % ) ,电泳型长型占优势 (96 % )。对HRV标本 ,再利用血清型特异的MAbELISA和 /或RT PCR进行G分型。结果表明 ,在 1998～ 1999年 ,在上述 8城市非细菌性腹泻流行季节 ,以HRVG1型为主要流行株 ,占阳性总数的 83 4% ,其次为G3(12 0 % )、G4(3 5 % )和G2 (3 2 % )。此外 ,有 3份(0 7% )HRV标本未能分型 ,12 (2 8% )份标本为混合感染。还结合 1982～ 1996年全国 12个地区 1382份HRV标本的分型资料 ,分析了我国HRVG血清型的流行规律。实验中又抽样选取了 12 4份G型HRV标本 ,用RT -PCR进行P分型 ,其中P[8]型 76份 (6 1 3% ) ,P[4 ]型 14份 (11 3% ) ,P[6 ]型 12份 (9 7% ) ,P[9]型 8份 (6 4% )。另外 15份 (12 1% )未能分出P型 ,有待进一步检定。实验中HRV分离株除了常见的P[8]G1(5 1 4% )、P[4 ]G2(4 6 % )毒株外 ,还检测到P[8]G3(11 0 % )、P[
Rotaviruses have been recognized as the major etiologic agents of acute gastroenteritis in infants and young children worldwide. Rotavirus serotype G (glycoprotein) and P (protease sensitive) are specified by two outer capsid proteins, VP7 and VP4, respectively. In this study, we examined 1,093 stool specimens collected in 1998～1999 rotavirus season of 8 regions in China from children with diarrhea. These specimens were screened for human rotavirus (HRV) by PAGE and/or ELISA and resulted in 396% (433) positives with more than 96% of long electropherotype. G serotype and P genotype of HRV were assigned by ELISA with MAbs and/or RT-PCR with type-specific primers respectively. Results showed that G1 (834%) was the most prevalent strain followed by G3 (120%), G4(35%), and G2(32%). Total 3 strains (07%) remained non-typeable. Mixed infections were rare (28%). Combined with 1982-1996 serotyping data of 1,382 HRV strains collected from 12 regions in China, the distributions of HRV G serotypes in different areas and in different years were analysed. 124 strains of G type specimens were selected for P genotyping. The results showed that P[8] was 613%, P[4] 113%, P[6]97% and P[9] was 64%. There were 15 strains (121%) remained non-typeable, which implied that some unique P types might exist. The G-P type combinations most frequently seen were P[8] G1 (514%) and P[4]G2 (46%), the other common combinations such as P[8] G3 (110%), P[8]G4 (64%), and other uncommon strains of the type as P[6] G1, P[6] G3, P[6] G4, and P[9] G1 were also observed. These results will provide a clear epidemiological background for development of rotavirus vaccine in China.
出处
《病毒学报》
CAS
CSCD
北大核心
2001年第1期17-23,共7页
Chinese Journal of Virology
基金
WHO资助! (V2 7/181/12 3 )
国家自然科学基金!(3 9870 0 3 2 )
