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莱克多巴胺、沙丁胺醇与克伦特罗三合一免疫亲和柱的制备及应用 被引量:3

Preparation and Application of Immunoaffinity Column for Ractopamine,Salbutamol and Clenbuterol
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摘要 将莱克多巴胺抗体和克伦特罗抗体偶联到溴化氰活化的琼脂糖凝胶上,制成免疫亲和柱,对其柱性能进行测试,并优化了使用条件,结合高效液相色谱-串联质谱对实际饲料样品进行检测。结果表明:该免疫亲和柱对莱克多巴胺、沙丁胺醇、克伦特罗的柱容量依次为49,27,24 ng/mL胶,保质期为5个月,最佳洗脱液为甲醇,对3种物质的回收率为78.9%~96.5%,RSD均小于1.5%,检出限为0.1~1 ng/g。该法可同时检测饲料中3种瘦肉精的含量。 The anti-ractopamine monoclonal antibody and anti-clenbuterol monoclonal antibody were both coupled with CNBr activated Sepharose 4B to make the immunoaffinity column. The column performance was tested and the use conditions were optimized by HPLC-MS /MS. The results showed that the column capacity to ractopamine,salbutamol and clenbuterol were 49,27,24 ng /mL glue,respectively,the storage time was five months,and the best eluent was methanol. The average recoveries of three lean meat essences ranged from 78. 9% to 96. 5% with relative standard deviations( RSD) lower than 1. 5%. The limits of detection( LOD) ranged from 0. 1 ng/g to 1 ng/g. The method could be applied in the simultaneous determination of the lean meat essence contents in feed samples.
出处 《分析测试学报》 CAS CSCD 北大核心 2014年第6期693-697,共5页 Journal of Instrumental Analysis
基金 南昌市生物资源保护和利用重点实验室项目(2012-SYS-04)
关键词 莱克多巴胺 沙丁胺醇 克伦特罗 免疫亲和柱 高效液相色谱-串联质谱 ractopamine salbutamol clenbuterol immunoaffinity column high performance liq-uid chromatography tandem mass spectrometry(HPLC-MS /MS)
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