摘要
目的探讨MRI对比剂钆双胺注射液（欧乃影；Gd—DTPA—BMA）应用后对家兔^99Tc^m-MDP骨显像的影响。方法采用自身前后对照方法，实验组（注射欧乃影＋^99Tc^m-MDP）与自身对照组（注射生理盐水＋^99Tc^m-MDP）处理相隔7d。（1）取家兔5只，注射欧乃影或生理盐水后30min注射^99Tc^m-MDP，并于注射^99Tc^m-MDP后5、10、30、60、120、180、240和360min、24h时行骨显像，勾画ROI，计算靶／本底（T／B）值，观察家兔显像剂分布及代谢情况。（2）取家兔30只，按欧乃影或生理盐水与^99Tc^m-MDP的注射间隔（30、60、120、240和360min、24h）分为6组，每组5只。均于注射^99Tc^m-MDP后120min行骨显像，勾画ROI，计算T／B值。（3）采用TLC测定2种药物相互作用后在体内的放化纯差异。以配对t检验进行统计分析。结果注射欧乃影后30min再注射^99Tc^m-MDP，5min时肝、脾开始显影，其T／B比值120min时达最高峰（肝、脾分别为4．56±0．32和3．56±0．41）；而2组椎体24h时T／B值均达最大值（实验组为4．32±0．07，自身对照组为6．31±0．09），但各时间点实验组均较自身对照组低。两药物注射间隔为30、60、120和240min时，可出现肝、脾异常显影，对应的实验组T／B（肝：2．47～4．22；脾：1．85～3．23）明显高于自身对照组（肝：1．52—1．58；脾：1．25～1．29），但实验组椎体的T／B（3．08～4．28）则较自身对照组低（4．82～4．85），以上差异均有统计学意义（t=7．750—31．916，均P〈0．05）。余时间间隔组，肝、脾未见明显摄取^99Tc^m-MDP。TLC曲线示，实验组在Rf为0—0．2处形成小峰，^99Tc^m-MDP放化纯降为（63．51±2．24）％。结论注射欧乃影后短时间内行^99Tc^m-MDP骨显像，肝、脾会出现异常摄取；间隔360min再行^99Tc^m-MDP骨显像可避免此现象发生。
Objective To investigate the effects of MRI contrast agent Omniscan on ^99Tc^m-MDP bone imaging in rabbits. Methods Self-control method was used in this study： experimental group was in- jected with Omniscan ＋ ^99Tc^m-MDP; control group was injected with normal saline ＋ ^99Tc^m-MDP. The time interval between 2 experiments was 7 d. （ 1 ） Five rabbits were injected Omniscan/normal saline and ^99Tc^m-MDP with the time interval of 30 min. Whole body bone imaging was performed to observe the distribution and metabolism of ^99Tc^m-MDP in the two groups at 5, 10, 30, 60,120, 240, 360 min and 24 h after injection of ^99Tc^m-MDP. ROI was drawn and T/B was calculated. （2）Thirty rabbits were divided into 6 groups according to the different time intervals （30, 60, 120,240 and 360 min, 24 h） between injection of Omniscan/ normal saline and ^99Tc^m-MDP. Whole body bone imaging was performed in the 6 groups 2 h after injection of ^99Tc^m-MDP. ROI was drawn and T/B was calculated. （3）The radiochemical purity in vivo was investigated by TLC. Paired t test was used to analyze the data. Results （ 1 ） When the injection interval of Omniscan and ^99Tc^m-MDP was 30 min, the radioactive uptake by liver and spleen was shown at 5 min, and the T/B ratios were the highest at 2 h （4.56±0.32 and 3.56±0.41 for liver and spleen respectively）. The T/B ratio of vertebra in experimental group was lower than that in control group at all time points and it reached the highest point at 24 h in both groups （（4.32±0.07） and （6.31±0.09） respectively）. （2）When the injection interval was 30, 60, 120, 240 min, the liver and spleen were visualized. The T/B ratios of liver and spleen were higher in experimental groups （ liver： 2.47- 4.22; spleen ： 1.85- 3.23 ） compared to those of the control group （liver： 1.52-1.58 ;spleen： 1.25-1.29） , and the T/B ratio of vertebra was lower in experimen- tal groups （ 3.08- 4.28 ） than that in control group （ 4.82 - 4.85 ; t = 7.750 - 31.916, all P〈 0.05 ）. Radioactive uptake by the liver and spleen was not shown in other injection interval groups. The TLC curve showed a small peak at Rf of 0-0.2 in the experimental group and the radiochemical purity of ^99Tc^m-MDP decreased to （63.51±2.24） %. Conclusions When Omniscan is injected before ^99Tc^m-MDP, liver and spleen may show abnormal radioactive uptake on bone imaging. Taking 360 min as the injection interval may help to avoid it.
出处
《中华核医学与分子影像杂志》
CSCD
北大核心
2014年第3期226-230,共5页
Chinese Journal of Nuclear Medicine and Molecular Imaging
基金
四川省医学重点学科建设项目（2008-16）
关键词
骨骼
放射性核素显像
造影剂
MDP
兔
Skeleton
Radionuclide imaging
Contrast media
MDP
Rabbits
