摘要
目的:探讨人类白细胞抗原G(HLA-G)在子痫前期患者血清及胎盘组织中的表达及意义。方法:收集子痫前期患者20例(观察组)和正常妊娠者20例(对照组)的血清及胎盘组织,采用酶联免疫吸附法(ELSIA)、实时荧光定量PCR(real-time RT-PCR)以及免疫蛋白印迹(Westen Blot)检测两组血清可溶性人类白细胞抗原G(sHLA-G)及胎盘组织中HLA-G的表达差异,并通过HE染色观察两组胎盘的病理变化。结果:①观察组血清及胎盘组织中HLA-G表达水平均低于对照组,两组差异均有统计学意义(P<0.05)。②观察组与对照组比较,胎盘绒毛间质血管数及绒毛数平均值减少(P<0.01,P<0.05),合体滋养细胞结节增多(P<0.05),绒毛间质纤维化及纤维素样坏死显著增多(P<0.01),细胞滋养细胞明显增生(P<0.01),干绒毛小动脉管腔直径明显变小(P<0.01)。③相关分析显示,血清中sHLA-G表达水平与胎盘中HLA-G蛋白表达水平呈正相关(r=0.909,P<0.05)。HLA-G mRNA及HLA-G蛋白表达强度与胎盘绒毛血管密度呈正相关(r=0.907,P<0.05;r=0.904,P<0.05)。结论:HLA-G可能与子痫前期的发生、病理生理过程密切相关。
Objective: Explore the expression of human leukocyte antigen-G (HLA-G)in the serum and placenta of preeclampsia patients. Methods: Enzyme-linked immunosorbent assay ( ELISA), real-time PCR and Western Blotting were used to detect HLA-G expression in serum and placenta tissue in 20 patients with preeclampsia ( experimental group) and 20 normal pregnant women ( control group). Pathological characteristics of placenta tissue was also compared between two groups. Results:(1) HLA-G in both serum and placenta decreased significantly in the preeclampsia group (P 〈 0. 05). (2)Compared with normal pregnant women,the amount of placenta villus stroma blood vessels ( P 〈 0. 01 ) and villus ( P 〈 0.05) decreased in patients with preeclampsia, which was characterized by increase of syneytiotrophoblast cells nodule( P 〈 0.05), increase of villus stroma fibrosis and fibrionoid necrosis ( P 〈 0. 01 ), increase of cytotrophoblastic cells ( P 〈 0.01 ), and decrease of villus arteride diameter( P 〈 0. 01 ). (3)There was a positive correlation between ser- um and placental HLA-G levels( r =0. 909, P 〈0.05). There was a positive correlation of the expression of either HLA-G mRNA or protein with the count of vessels in placental villus( r=0. 907 and 0. 904, P〈0. 05). Conclusions:The expression of HLA-G decreased significantly in serum and placental tissue of preeclampsia patients,which may be correlated with the physiological process of preeclampsia.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2014年第6期452-456,共5页
Journal of Practical Obstetrics and Gynecology