摘要
目的 建立普洱茶中黄曲霉毒素B1（AFB1）的免疫亲和柱净化-酶联免疫吸附检测方法。方法 粉碎后的普洱茶样品经三氯甲烷提取,水浴挥干后用甲醇-PBS溶解,样品液通过黄曲霉毒素B1免疫亲和柱净化洗脱,在450nm波长使用酶联免疫试剂盒（ELISA）定量检测黄曲霉毒素B1含量。结果 该方法在2.0~100.0μg/kg浓度范围内百分吸光率和浓度对数呈良好的线性关系,相关系数达0.999 8,样品最低检出限为1.0μg/kg,重复测定相对标准偏差为4.72%,3个水平（1.0、10.0、50.0μg/kg）的样品加标回收率为83.00%~104.90%。结论 本法具有简单、快速、高效等优点,适用于普洱茶复杂样品的AFB1定量检测。
Objective To establish a method combining affinity column purification and enzyme linked immunosorbent as- say （ELISA） for detection of aflatoxin B1 （AFB1） in the Pu＇er Tea. Methods Ground samples of Pu＇er tea were first extracted with chloroform. And then extracted solutions were re-dissolved with methanol-PBS after dried-up the extraction solvent. The sample solution was then cleaned up by AFB1 specific-affinity column purification. Finally, the AFB1 was measured with ELISA at a wavelength of 450 nm. Results This method had a linear relationship between percentage absorbanee and logarithm concentration （ r = 0. 999 8） at the concentration range of 2.0 to 100.0 μg/kg. The detection limit was 1.0μg/kg and theRSD was 4.72%. The range of recoveries was 83.0%-104.90%. Conclusion The method is simple,rapid and effective,and it is suitable for the quantitative measurement of AFB1 in the Pu＇er tea.
出处
《预防医学论坛》
2014年第6期413-415,共3页
Preventive Medicine Tribune
基金
广州市荔湾区科技计划项目(编号:20121213027)
