摘要
目的在实验室酶法提取的基础上,优化苦豆草生物碱酶法提取小试工艺条件。方法以苦豆草生物碱的提取率为指标,在小试条件下优化提取次数、料液比、提取时间,并与传统提取法比较。结果酶法提取最佳的小试工艺条件为:酶解时间4h,溶剂pH=6,温度为50℃,加酶量为1∶1 000,酶解后,加入盐酸至浓度为4mL·L-1,料液比1∶16,提取2次,第1次2h,第2次1h。苦豆草生物碱的提取率达到2.17%结论实验室提取工艺与小试生产存在差异,经条件优化基本达到实验室提取生物碱的提取率。
Objective Based on the laboratory enzymatic extraction method,to optimize small-scale process conditions of alkaloids enzymatic extraction fromSophora alopecuroides L..Method By using the alkaloids extraction rate as index,extraction time,solid-liquid ratio and extraction times were optimized in small-scale process,and the results were compared with the traditional extraction method.Results The optimal small-scale process conditions of enzymatic extraction were as follows：solvent pH 6,amounts of enzyme（1∶1 000）,enzymatic hydrolysis at temperature 50℃for 4h.Then hydrochloric acid was added to the concentration of 4mL·L^-1,solid-liquid ratio 1∶16,extracting for 2times.The first time extracted 2h,and the second time extracted 1h.The extraction rate of alkaloids fromSophora alopecuroides L.was 2.17%.Conclusion Although the small-scale technology was different from the laboratory enzymatic extraction,the extraction rate was almost same.
出处
《西北药学杂志》
CAS
2014年第4期347-350,共4页
Northwest Pharmaceutical Journal
基金
2012年宁夏高等学校科学技术项目(编号:NGY2012125)
关键词
苦豆草
生物碱
酶法提取
Sophora alopecuroides L.
alkaloids
enzymatic extraction
