摘要
本实验建立了高效液相色谱法（HPLC）测定饲料中癸氧喹酯含量的方法。该方法采用C18色谱柱（250 mm×4.6 mm，粒径5μm），以0.025 mol/L氯化钙溶液＋甲醇（15∶85，V/V）为流动相，激发波长为326 nm，发射波长为384 nm，流速为1.0 mL/min，外标法定量。结果表明，该方法最低检测限为0.5 mg/kg，癸氧喹酯的浓度为0.02～2.0 mg/L时，峰面积与浓度线性关系良好（r=0.99994），平均回收率在80%以上，变异系数小于10%，稳定性、重现性好，可用于饲料中癸氧喹酯的含量测定。
This experiment was conducted to study the method for high performance liquid chromatographic （HPLC） for determination of decoquinate in feeds by HPLC method.A C18 column （250 mm×4.6 mm,5μm）was used with the mobile phase of 0.025 mol/L calcium chloride-methanol （15∶85,V/V）at the excitation wavelength of 326 nm and emission wavelength of 384 nm with a flow rate of 1.0 mL/min.The quantification was carried out by the external standard method. The limit of quantitation（LOQ）was 0.5 mg/kg.The calibration curves had good linear within 0.02 - 2.0 mg/L（r=0.99994）. The average recovery was above 80%.The variation coefficient was less than 10%.The results showed that the method had advantages of good stability and reproducibility,and could be used for the determination of decoquinate in feed.
出处
《中国饲料》
北大核心
2014年第13期37-38,42,共3页
China Feed
关键词
癸氧喹酯
饲料
高效液相色谱法
decoquinate
feed
high performance liquid chromatographic method
