摘要
目的:探讨miR-19对肺腺癌A549细胞株迁移能力的作用。方法:运用qRT-PCR检测miR-19在肺上皮细胞株BEAS-2B和肺腺癌A549-Luc细胞株中的表达;建立稳定过表达miR-19的A549-Luc肺癌细胞株;qRT-PCR鉴定稳定细胞株A549/RFP+/H2B和A549/RFP+/m19中miR-19的表达水平;观察其形态变化及Transwell小室细胞迁移实验检测细胞迁移能力变化。结果:miR-19在BEAS-2B及A549-Luc细胞中的表达差异具有显著性(t=-20.954,P<0.001)。成功建立了稳定过表达miR-19的肺腺癌细胞株A549/RFP+/m19;观察发现A549/RFP+/m19细胞形态发生变化,且与空载A549/RFP+/H2B细胞相比,A549/RFP+/m19细胞迁移能力增强(P<0.001)。结论:miR-19可使肺腺癌A549-Luc细胞迁移能力增强。
Objective To investigate the effects of miR-19 on the migration ability of lung adenocarcinoma cell line A549. Methods The expressions of miR-19 in lung epithelial cell line BEAS-2B and lung adenocarcinoma cell line A549-Luc were detected by qRT-PCR.The A549-Luc cell line which over-expressed miR-19 was established. The expression levels of miR-19 in A549/RFP＋/H2B and A549/RFP＋/m19 were identified by qRT-PCR. The morphology of A549/RFP＋/m19 was observed,and the migration ability of A549/RFP＋/mI9 was detected by transwell migration assay. Results The expression levels of miR-19 differed significantly between BEAS-2B cells and A549-Luc cells （t = -20.954, P 〈 0.001 ）. The lung adenocarcinoma cell line A549/RFP＋/ m19 which over-expressed miR-19 was successfully established. Changes in A549/RFP＋/m19 cell morphology were found. As compared with A549/RFP＋/H2B ceils, A549/RFP＋/m19 had an increased migration ability （P 〈 0.01 ）. Conclusions miR-19 enhances the migration ability of lung adenocarcinoma A549-Luc cells.
出处
《实用医学杂志》
CAS
北大核心
2014年第13期2031-2034,共4页
The Journal of Practical Medicine
基金
国家自然科学基金青年科学基金项目(编号:81302965)
广东省自然科学基金博士启动项目(编号:S2013040014341)
广东省医学科研课题(编号:B2013061)
