摘要
目的测试两种装载方法实验动物垫料压力蒸汽灭菌的条件,并比较灭菌效果。方法分别用笼盒重叠法和袋装法装载刨花垫料,121℃、20 min压力蒸汽灭菌,用化学指示卡测试灭菌条件。分别取两种装载方法灭菌前后的垫料10 g放入灭菌生理盐水中充分摇匀,取1 ml接种于血平板,37℃、48 h培养,以血平板上是否生长菌落为灭菌合格与否的判定标准。结果与标准色比较,笼盒重叠法未能全部达到灭菌条件,其灭菌合格率为83.3%;袋装法各部位均达到灭菌条件,其灭菌合格率为100.0%。结论袋装法灭菌效果优于笼盒重叠法。
Objective To test the pressure steam sterilization conditions using two packing methods of laboratory animal bedding, and compare the effect of sterilization. Methods Cages overlap methods and bagged methods were used respectively to packing animal bedding, pressure steam sterilization at 121% for 20 minutes with conditions tested by chemical indicator card. 10g bedding before and after the sterilization respectively using two 1 plate oading methods were taken and dissolved into sterile saline, then take 1 ml of it and inoculated on blood at 37 ℃ for 48 hours, whether there is colony grown in blood agar is the decision criteria for sterilization is qualified or not. Results Compared with the standard color, not all animal bedding packing with cages overlap method was sterilized effectively, the effectively sterilized rate was 83.3% ; All animal bedding packing with bagged method were sterilized effectively, the effectively sterilized rate was 100%. Conclusions The steri- lization effect of animal bedding packing with bagged method is better than cages overlap.
出处
《医学动物防制》
2014年第6期633-634,共2页
Journal of Medical Pest Control
基金
重庆市科委资助项目(cstc2012ggB10005)
关键词
垫料
灭菌
化学指示卡
实验动物
Bedding
Sterilization
Chemical indicator
Laboratory animal
