摘要
目的建立凹叶厚朴Magnolia officinalis subsp.biloba快速繁殖体系。方法以选育的优质凹叶厚朴种胚为初始外植体,采用单因素及正交试验筛选不同基本培养基,不同植物调节剂(6-BA、NAA、IBA、IAA),获取最适种胚启动培养基,丛芽增殖培养基和生根培养基。结果适宜种胚萌发的启动培养基为B5+6-BA 0.5 mg/L+NAA 0.1 mg/L,出芽率达87.0%;适合丛芽增殖的培养基为MS+6-BA 2.5 mg/L+NAA 0.5 mg/L,增殖系数可达6.2;适合生根的培养基为1/2 MS+NAA 0.5mg/L,30 d生根率可达84%。结论建立了凹叶厚朴快速繁殖体系,为凹叶厚朴优质种苗的工厂化育苗奠定了基础。
Objective To establish a rapid propagation system of Magnolia officinalis subsp, biloba. Methods Using peeling seeds ofM. officinalis subsp, biloba as the initial explants, different media and various combinations of plant growth regulators （6-BA, NAA, IBA, and IAA） on the seeds germination, the seedlings subculture, and rooting culture were studied by single factor test and orthogonal test. Results The best culture medium of seeds germination was B5 ＋ 6-BA 0.5 mg/L ＋ NAA 0.1 mg/L, and the budding percentage was 87.0%; The effective medium for cluster buds-inducing and subculture was MS ＋ 6-BA 2.5 mg/L ＋ NAA 0.5 mg/L, and the propagation coefficient was 6.2; The best rooting medium was 1/2 MS ＋ NAA 0.5 mg/L, and the rooting rate was over 84% after 30 d. Conclusion Rapid propagation technique system is established in order to lay technique foundation for the industrial production of M. officinalis subsp, biloba.
出处
《中草药》
CAS
CSCD
北大核心
2014年第12期1769-1774,共6页
Chinese Traditional and Herbal Drugs
基金
林业公益性行业科研专项经费"金银花
凹叶厚朴新品种创制及利用技术研究"(201104023)
湖南省教育厅重点项目(2012A0146)
关键词
凹叶厚朴
组织培养
快速繁殖
培养基
正交试验
Magnolia officinalis subsp, biloba （Rehd.et Wils.） Law.
tissue culture
rapid propagation
medium
orthogonal test
