摘要
目的利用静电纺丝技术制备丹参素-聚己内酯(DSS-PCL)纳米纤维支架。方法以二氯甲烷-N,N-二甲基甲酰胺(CH2Cl2-DMF)8∶2为溶剂,利用静电纺丝仪制备载丹参素的纳米纤维,通过扫描电镜(SEM)观察纤维形貌,通过体外释放实验考察载药纳米纤维支架中丹参素的释放规律,通过MTT法和溶血率对载药纳米纤维支架的安全性进行初步评价。结果 CH2Cl2-DMF混合溶剂体系下药物和材料都能够很好地溶解并顺利电纺得到纳米纤维,平均纤维直径分别为210 nm和190 nm,体外释放实验表明药物的释放经过一个快速释放后进入缓慢释放。MTT法测得PCL及载有丹参素的纳米纤维对L929细胞存活率并无明显影响,溶血率实验测得各组纳米纤维的溶血率低于5%。结论静电纺丝法制备DSS-PCL纳米纤维的工艺简单易行,易于推广。
Objective To prepare Danshensu loaded polycaprolactone（DSS-PCL） nanofiber scaffold by using electrospin technology.Methods Using dichloromethane-N,N-dimethyl formamide（CH2Cl2-DMF） 8∶2 as solvent,nanofibers containing danshensu was prepared by electrospinning,fiber morphology was observed by SEM,danshensu in vitro release rule from drug loaded nanofiber scaffold was investigated,and the security of nanofiber was assessed by MTT and hemolysis test.Results By using CH2Cl2-DMF as solvent,drug and materials could be well dissolved and successfully electrospun into nanofiber,the average fiber diameters were 210 and 190 nm,the in vitro release experiment showed that the drug release after a quick release into the slow release.MTT test indicated that PCL and DSS-PCL nanofiber showed no influence to the cell viability,and hemolysis test showed hemolysis ratio of nanofiber was under 5%.Conclusion DSS-PCL nanofibers is easy to prepare by electrospinning.
出处
《中草药》
CAS
CSCD
北大核心
2014年第13期1850-1854,共5页
Chinese Traditional and Herbal Drugs
基金
广东医学院面上项目(XK1203)
博士启动项目(XB1303)
湛江市科技攻关计划项目(2012C3106015)
