摘要
目的 研究未致明显急慢性损伤剂量的超声能否增强阿霉素对卵巢癌细胞的细胞毒作用。方法 分 3组以克隆原细胞分析法分析各组细胞对阿霉素的反应性 ,仅以阿霉素处理组 (对照组 ,ADM)、超声辐照前给药组 (ADM +US)、超声辐照后给药组 (US +ADM)。并以荧光分析法测定 3组细胞细胞内药物聚积量。结果 ADM组、ADM +US组和US +ADM组IC50 分别为 0 .0 0 83 μg/ml、<0 .0 0 1μg/ml和0 .0 0 65 μg/ml,ADM +US组、US +ADM组克隆存活率均较ADM组低。与对照组比较 ,ADM +US组与US +ADM组克隆存活率降低 (P <0 .0 0 1) ,且ADM +US组克隆存活率明确低于US +ADM者。ADM +US组细胞内药物聚积量较ADM组高 (P <0 .0 1) ,US +ADM组细胞内药物聚积量未明显增加 (P >0 .0 5 )。结论 低频超声可增强阿霉素对卵巢癌细胞细胞毒作用 。
Objective To determine whether the ultrasound, at a dosage that didn't lead to acute and delayed inhibition, could enhance the cytotoxicity of adriamycin to human ovarian carcinoma cell line 3AO in vitro. Methods Drug sensitivity was analyzed by clongenic assay. Cells were treated by adriamycin single in group ADM(control), adriamycin prior to ultrasound exposure in group ADM+US and ultrasound irradiation prior to adriamycin administration in group US+ADM. The intracellular drug accumulations in each group were determined by flurorimetry. Results The values of IC 50 were 0.0083 μg/ml, < 0.001 μg/ml and 0.0065 μg/ml in group ADM, ADM+US and US+ADM respectively. The clone surviving rates in group ADM+US and US+ADM were decreased (P< 0.001 , P< 0.01 ), compared with control. The surviving rates in ADM+US were also less than those in group US+ADM (P< 0.01 ). The intracellular drug accumulations in group ADM+US were promoted (P< 0.01 ),but not significantly in group US+ADM (P> 0.05 ). Conclusion The low frequency ultrasound could potentiate the cytotoxicity of adriamycin to human ovarian carcinoma cells and the promoted intracellular drug contents played the major role.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2001年第1期26-28,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
"九五"国家科技重点攻关资助项目! (96 90 5 0 2 0 1 )
