摘要
目的探讨浅Ⅱ度烧伤创面愈合过程中相关生长因子及其受体的作用。方法采用大鼠浅Ⅱ度烫伤模型,观察了大鼠浅Ⅱ度烫伤后创面愈合过程中组织学、炎性细胞、表皮细胞周期的变化,检测了创面相关生长因子及受体的基因表达。结果炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞;表皮细胞周期变化为伤后3天细胞增殖活跃,伤后7天细胞分裂明显增多;伤后1,3天 PDGF(血小板源性生长因子)、PDGFR(血小板源性生长因子受体)和 EGFR(表皮生长因子受休)基因表达明显增强,伤后3,5天 EGF(表皮生长因子)、TGFβ-R_2(转化生长因子β受体α)基因表达增强,伤后5,7天 TGFβ-R_1(转化生长因子β受体1)基因表达增强,伤后7,10天 TGFβ_1(转化生长因子β)基因表达增强。结论烧伤诱导相关生长因子及受体的基因表达,并且具有时相性和可逆性;相关生长因子基因表达与表皮细胞周期间可能存在相互调控作用,生长因子是调控创面愈合的主要因素。
Objective To investigate the role of growth factors and their receptors in partial-thick- ness burn wound healing.Methods SD rats were used.After 10% total body surface area partial-thick- ness burn,wound tissues were harvested on postburn days(PBDs)0(normal control),1,3,5,7,10 and 14 respectively.The gene expressions of growth factors and their receptors were determined in wound by in situ hybridization and slot blotting hybridization.At the same time,the process of the wound healing was observed histologically,and the regeneration cycle of epidermal cells and the temporal change in in- flammatory cells were measured.Results Inflammatory cells infiltrated into wound surface were neu- trophils,followed by macrophages and lastly lymphocytes.Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn.The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R_2 were highest on PBD 3.In addition,the gene expression of TGFβ-R_1 and TGFβ_1 increased significantly on PBDs 5 and 7 respectively.Conclusion The data suggested that burn can induce gene expression of EGF,PDGF, TGF-β_1 and their receptors temporally,spatially,and reversiblity,which might play a major role in burn wound healing,and the mutual regulation may exist in the gene expression and the cell cycle.
基金
本课题受国家自然科学基金资助
关键词
烧伤
创面愈合
增殖周期
生长因子
受体
Burns
Wound healing
Cellular cycle
Growth factors and receptors
