摘要
目的 探讨生长抑素对肝癌细胞血管内皮生长因子 (VEGF)表达的影响。方法 将 2 0只小鼠随机分为 2组 ,每组各10只。每只小鼠于左腋窝皮下注射小鼠肝癌细胞株细胞。A组为实验组 ,皮下接种肿瘤 2 4h后予奥曲肽 (10 0 ug·kg- 1·d- 1 )腹腔注射 ,连用 14d;B组为对照组 ,予以相同容积的无菌生理盐水腹腔注射 ,连用 14d。 14d后处死小白鼠 ,检测肿瘤大小、血管内皮生长因子 (VEGF)。结果 皮下接种 7d天及 14d天后 A组皮下肿瘤的体积均明显小于 B组 (P<0 .0 5 ,P<0 .0 1) .A组 VEGF表达明显低于 B组 ,并统计学差异有显著性 (Ridit检验 ,P<0 .0 5 ) ;A组 MVD亦较 B组为低 ,但统计学差异无显著性 (P=0 .0 75 )。结论 生长抑素对小白鼠肝癌移植瘤具有抑制作用 ,对肿瘤 VEGF的抑制作可能为其机理之一 ,对肿瘤组织血管形成的抑制作用亦可能为其原因。
Objective To evaluate the effect of somatostatin on the expression of VEGF in HCC.Meghods 20 male mice were enrolled into this study.They were randomized into two groups,each group contained 10 mice.HCC cell line was implanted subcutaneously into left armpit of each mice.Group A is experimental group,everyone received octreotide (100 μg·kg -1 ·d -1 ×14 days)intraperitoneal injection after implantation.Group B is control group,each mouse in group B received same amount of saline solution intraperitoneal injection .Subcutaneous tumor's size was measured everyday.14 d after implantation,all mice were killed,and the subcutaneous tumors were removed for immunohistochemical examination of VEGF.Results Mean size of subcutaneous tumors in Guoup A is significantly smaller than that in Group B in 7 days and 14 d after implantation respectively.The expression of VEGF in Group B is significant higher than that in Group A (Ridit analysis,P<0.05).MVD in Group B is greater,but without statistic significant difference (P>0.05).Conclusion Somatostatin can inhibit subcutaneous transplantation model of H 22 cell line in mice.Inhibition of tumor angiogenesis may be one of the mechanisms of its antitumor effect.
出处
《肝胆外科杂志》
2001年第1期53-54,共2页
Journal of Hepatobiliary Surgery
关键词
生长抑素
肝细胞癌
血管密度
血管内皮生长因子
Somatostatin
Hepatocellular carcinoma
Microvesseldensity
Vascular endothelial growth factor
