摘要
目的 :比较免疫抑制鼠和正常鼠根尖周炎中IL - 1α、TNF -αmRNA表达 ,了解免疫抑制对根尖周炎的影响。方法 :2 0只SD大鼠分成实验组 (腹腔注射环磷酰胺 )和对照组 (腹腔注射生理盐水 ) ,3周后将实验组和对照组上、下颌磨牙开髓 ,分别于术后 3、7、14、2 8d取根尖周组织 ,用RT -PCR方法检测IL - 1α和TNF-αmRNA表达 ,并进行半定量分析。结果 :实验组术后 7d根尖周组织中即有IL - 1α和TNF -αmRNA表达 ,14d达高峰 ,2 8d有所下降 ;对照组呈与此相似的动态过程 ,两者间的表达量无明显差别。结论 :外周血白细胞减少对根尖周炎中IL - 1α表达无明显影响。
AIM: To compare the expression of IL-1α and TNF-α mRNA in periapical disease between rats with immuno-suppression and rats without treatment to investigate the effect of immuno-suppression on periapical disease. METHODS: Cyclophosphamide, an immuno-suppressant, was injected into the abdominal cavity of rats and the expression of IL-1α and TNF-α mRNA was examined with RT-PCR. RESULT:The expression of IL-1α and TNF-α mRNA was detected in periapical tissues at 7 th day and formed a peak at 14 th day and slightly decreased at 28 th day after the injection of the cyclophosphamide. There was no significant difference between the test and control groups. CONCLUSION: Decrease of the peripheral leucocytes has no effect on periapical disease. [
出处
《牙体牙髓牙周病学杂志》
CAS
2001年第2期92-94,共3页
Chinese Journal of Conservative Dentistry
