摘要
在MS基本培养基中添加不同植物生长调节剂（mg/L）对绿巨人无菌苗的叶柄进行离体培养，结果表明，MS + 6-BA 1.0 + NAA 2.0 + 2,4-D 1.0诱导效果最好，产生愈伤组织并分化出丛芽；MS + 6-BA 2.0～3.0 + NAA 0.2 + 0.2% PVP对芽的增殖效果好；附加0.2%PVP对防止褐变有一定效果；生根培养基选用1/2MS + NAA 0.1～0.5 + IBA 1.0的组合。
Petioles of Spathiphyllum floribundum cv. Maura Loa were cultured on MS medium supplemented with different concentration hormones. The results showed that MS medium containing 6-BA at 1.0mg/L, NAA at 2.0mg/L and 2,4-D at 1.0mg/L gave best results in callus induction; a combination of MS with 6-BA at 2.0～3.0mg/L, NAA at 0.2mg/L and 0.2% PVP was good for proliferation of adventitious buds; MS supplemented with 0.2% PVP could prevent from browning; and 1/2MS medium containing NAA at 0.1～0.5mg/L and IBA at 1.0mg/L was favorable for rooting.
出处
《亚热带植物科学》
2001年第1期32-35,共4页
Subtropical Plant Science
关键词
绿巨人
叶柄
离体培养
植株再生
培养基
植物生长调节剂
：Spathiphyllum floribundum cv. Maura Loa
petiole
tissue culture
plantlet regeneration
