摘要
目的 了解低水平血清乙肝病毒表面抗原 (HBsAg)的人群分布及其相关乙肝病毒血清标志物模式特征。方法 微粒子酶免疫分析技术测定 80 89例非肝炎病区住院患者血清HBsAg及其表面抗体 (抗 HBs)、乙肝病毒e抗原及其e抗体 (HBeAg、抗 HBe)和乙肝病毒核心抗体 (抗 HBc) ;根据定值参比血清和样本的HBsAg荧光速率值 阴性对照荧光速率值之比值 (S N值 ) ,并结合中和试验结果 ,确定浓度在 5 μg L以下的HBsAg阳性例数 ,并分析相关乙肝病毒 (HBV)血清标志物模式。 结果 共检出HBsAg阳性 816例 ,HBsAg浓度在 5 μg L以下的有 189例 ,占总数的 2 .34% ,占HBsAg阳性人群的 2 3 .16 %。其中 ,HBsAg浓度在 1μg L以下的有 84例 (4 4.40 % ) ;1～ 2 μg L的有 33例 (17 5 % ) ;2～ 5μg L的有 72例 (38.10 % )。对上述低水平HBsAg人群的 5项HBV血清标志物检测获得 8种模式 ,以“HBsAg、抗 HBc、抗 HBe阳性” ,“HBeAg和抗 HBs阴性”模式为主 (74.6 0 % ) ;累计HBsAg和抗 HBc同时阳性者占 94.17% ;HBsAg与抗 HBs同时阳性只出现在HBsAg 1μg L以下人群中 (6 45 % )。 结论 低水平血清HBsAg人群不容忽视 ,提高HBsAg检测灵敏度有重要意义 ;同时检测相关HBV血清标志物 。
Objective To investigate the distribution of the population with low level HBsAg in serum and the characteristics of their HBV serologic makers. Methods We detected HBsAg,HBeAg,anti HBc,anti HBe, and anti HBs in the serum samples from 8?089 non hepatic inpatients and defined the patients with HBsAg concentration equal to or less than 5 μg/L by using AxSyM MEIA system. Results 189 patients with HBsAg equal to or lower than 5 μg/L (2.34% of all 8?089 people and 23.16% of 816 HBsAg positive people) included 84(44.40%), 33(17.50%) and 72(38.10%) with HBsAg equal to or less than 1 μg/L, 1～2 μg/L and 2～5 μg/L respectively. Most of these patients were detected with “positive for HBsAg, anti HBc and anti HBe but negative for HBeAg and anti HBs”, and anti HBc antibodies were frequently present in their sera. Conclusion The population with low level HBsAg in serum should not be neglected and it is important to improve the test sensitivity of HBsAg and to detect the other HBV serologic markers.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2001年第1期39-41,共3页
Chinese Journal of Laboratory Medicine
关键词
肝炎表面抗原、病毒
免疫酶技术
Hepatitis B surface antigen
Immunoenzyme techniques
