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应用PAP免疫组化染色法观察输精管结扎16~28月家兔的睾丸组织学变化

Observation of hjstological changes of testes in vasectomized rabbits with PAP staining
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摘要 日本大耳白雄兔16只,行双侧输精管结扎手术。术后16、22、28个月处死动物,睾丸切片HE染色尤镜观察,大部分曲细精管恢复正常的成精过程,各级精细胞形态无明显异常,并可见呈灶状分布的姜缩曲细精管,被结缔组织包绕,有淋巴细胞和浆细胞浸润。PAP染色显示,恢复正常生精过程的曲细精管均阴性染色,萎缩的曲细精管与基底膜呈阳性染色。提示输精管结扎术后曲细精管萎缩与免疫损伤有关。 Sixteen rabbits were bilaterally vasectomized and investigated at intervals of 16, 22 and 28 months.The results showed that most seminiferous tubules recovered to normal histologically, and spermatogenesis also restored to normal, with only a few seminiferous tubules seen atrophied and surrounded by fibrous tissue. PAP staining showed that seminiferous tubules whose spermatogenesis recovered to normal were staining negative, while the atrophied tubules and their basement membranes were staining positive.
出处 《白求恩医科大学学报》 CSCD 1990年第1期37-39,共3页 Journal of Norman Bethune University of Medical Science
关键词 免疫组化 输精管结扎 睾丸 Immuno-histochemistry Vasectomy Testes
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参考文献3

  • 1赵雪俭,生殖与避孕,1989年,9卷,4期,264页
  • 2赵雪俭,白求恩医科大学学报,1986年,12卷,5期,383页
  • 3王忠山,白求恩医科大学学报,1985年,11卷,2期,91页

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