摘要
目的 分析戊型肝炎病毒 (HEV)Ⅳ型的全基因序列以比较与其他基因型的差异。方法 设计PCR引物对全基因进行分片段扩增 ,并对两个末端采用末端快速扩增方法 (RACE)进行扩增 ,对扩增产物进行克隆及测序。结果 HEV T1全序与Ⅰ型、Ⅱ型和Ⅲ型的核苷酸同源性分别为(74.8～ 75 .5 ) %、74.5 %和 (75 .3～ 76 .3) %。ORF1与已报道的Ⅰ型、Ⅱ型和Ⅲ型氨基酸同源性分别为(85 .0～ 86 .7) %、85 .0 %和 (88.5～ 88.7) % ;ORF2与已报道的Ⅰ型、Ⅱ型和Ⅲ型的氨基酸同源性分别为 (91.6～ 92 .4) %、90 .1%和 (91.9～ 93.0 ) % ;ORF3与已报道的Ⅰ型、Ⅱ型和Ⅲ型的氨基酸同源性分别为 (75 .9～ 77.8) %、75 .0 %和 (79.6～ 83.3) %。结论 这一研究为今后发展戊型肝炎诊断试剂及戊型肝炎疫苗提供了新的、重要的分子生物学基础。
Objective To analyze the full length sequence of HEV genotype Ⅳ and compare it with that of HEV genotype Ⅰ, Ⅱ and Ⅲ. Methods The fragments of HEV genotype Ⅳ were amplified with RT PCR and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned and sequenced. Results The full length sequence of HEV T1 showed homogeneous of 74.8% 75.5%, 74.5% and 75.3% 76.3% with HEV genotype Ⅰ, Ⅱ and Ⅲ respectively. ORF1 protein of HEV T1 has 85.0% 86.7%, 85.0% and 88.5% 88.7 % identical to HEV genotype Ⅰ, Ⅱ and Ⅲ respectively with amino acid composition. ORF2 protein of HEV T1 is homogenous by 91.6% 92.4%, 90.1% and 91.9% 93.0% to HEV Ⅰ, Ⅱ and Ⅲ respectively and ORF3 has 75.9% 77.8%, 75.0% and 79.6% 83.8% identical to HEV Ⅰ, Ⅱ and Ⅲ respectively. Conclusion This study provides the new molecular basis for the development of HEV diagnostic kit and vaccine.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2001年第2期210-213,共4页
Chinese Journal of Microbiology and Immunology
基金
英国WellcomeTrust基金组织资助
