摘要
目的 利用逆转录聚合酶链反应 (RT PCR)法在核酸水平检测并证实肝豆状核变性 (Wilson病 ,WD)基因可以在Me32aT2 2细胞株中得到有效表达 ,为以后更深入的研究奠定基础。方法 采用脂质体转染法将WDcDNA重组真核表达载体pRc/CMV WD导入无血清的Me32aT2 2 /2L细胞株 ,RT PCR在核酸水平检测WD基因在Me32aT2 2 /2L细胞株的表达。结果 在转染的Me32aT2 2 /2L细胞株中可检测到WDcDNA表达 ,而在未转染的Me32aT2 2 /2L细胞中RT PCR检测结果呈阴性。结论 WD基因转染至Me32aT2 2
Objective Determination of Wilson disease gene mRNA expression in human fibroblast cell strain (Me32aT22/2L) by reverse transcription polymerase chain reaction (RT PCR). Methods Using lipofection reagent, the plasmid vector carrying the Wilson disease gene (pRc/CMV WD) was transferred into Me32aT22/2L cultured in serum free complement medium. RT PCR was used to determine WD mRNA expression in Me32aT22/2L. Results Wilson disease gene expression was detected in Me32aT22/2L, while no specific signals were detected in untransfected fibroblast. Conclusions It demonstrated that Me32aT22/2L strain could express the Wilson disease gene, suggesting that Wilson disease gene transfer might develop a new approach to study Wilson disease.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2001年第2期83-85,共3页
Chinese Journal of Nervous and Mental Diseases
基金
卫生部临床学科重大项目 !(编号 :370 91)
中山医科大学"2 11工程"重点建设项目
广东省自然科学基金 !(编号 :96 0 12 8)
广东
